Cell Information:

Lung fibroblasts play an important role in airway inflammation and airway remodeling. Human Lung Fibroblast (HLF) has been used as an important tool for medical and pharmaceutical studies. For examples: HLFcan be a target for asthma medications in pharmaceutical development; Fibroblast -based assay was used for screening of new antimicrobial drugs; Human cytomegalovirus (HCMV) was found to completely down-regulate EGF receptor on the surface of fetal HLF; Pseudomonas autoinducer N-(3-oxododecanoyl) homoserine lactone induces cyclooxygenase-2 and prostaglandin E2 production in HLF important for inflammation and immune defenses; Studies also show cigarette smoke extract added to the medium induces growth inhibition of HLF.

The Transfection Reagent:

Transfection of primary cells, such as HLF, has always been challenging, both technically and financially. The most common systems currently used for molecular delivery to these cells include expensive electroporation instruments and relatively toxic chemical formulations.

Human Lung Fibroblast (HLF, NHLF) Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting HLF. The proprietary formulation of lipids and polymersensures the highest possible transfection efficiencies and viabilities for HLF. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Specifically optimized to deliver nucleic acids into HLF
• Highest efficiency to ensure experimental success
• Extremely gentle to cells
• 0.5 ml is able to transfect about 1000 wells of 24-well plate
• Deliver single or multiple plasmids
• Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
• Compatible with serum
• Compatible with transfection in any plate formats
• Economical: High efficiency means less amount of nucleic acid & reagent is needed
• Developed and manufactured by EZ Biosystems

EZBiosystems美国EZBiosystems™公司是一家全球性转染试剂和基因表达生产服务提供商，公司的研发宗旨是让生命科学研究变得更简单，快速，容易！该公司主要产品之一为新一代转染试剂Avalanche 系列产品，该产品集合了组合化学，分子生物学和细胞生物学技术。公司致力于开发针对不同细胞类型的专用转染试剂。公司研发团队由多个学科经验丰富的专家组成，包括组合化学、脂类化学、生物物理学、分子生物学和细胞生物学。目前,EZBiosystems™主要提供广谱性基因及siRNA体外转染试剂、体内转染试剂、生物治疗性蛋白表达专用转染试剂以及150余种细胞特异性转染试剂。

EZBiosystems特约代理EZBiosystems品牌名称：EZBiosystemsEZBiosystems品牌logo：EZBiosystems品牌官网：http://ezbiosystems.com/EZBiosystems品牌链接：http://www.annoron.com/brand-342.htmlEZBiosystems品牌介绍：EZ生物系统™是一家全球性的基因转染和基因表达产品和服务提供商。EZBiosystems™isaworldwideprovideroftransfectionandgeneexpressionproducts&services.OneofthemajorfocusesofEZBiosystems™hasbeentheresearch,development,andmanufactureofnext-generationtransfectionreagents----theAvalanche®TransfectionReagentseriesbyapplyingourexpertiseincombinatorialchemistry,molecularBIOLOGy,andcellbiology. EZBiosystems品牌相关产品：品牌货号名称规格EZ BiosystemsEZT-CRPR-1Avalanche®-CRISPR Transfection Reagent0.5ml 1.5mlEZ BiosystemsEZT-OMNI-1Avalanche®-Omni Transfection Reagent0.75ml 1.5ml5*1.5mlEZ BiosystemsEZT-EVDY-1Avalanche®-Everyday Transfection Reagent0.75ml 1.5ml