Overview:
SIRT6 is a member of the sirtuin family of proteins which are homologs to the yeast Sir2 protein. Sirtuin family contain a sirtuin core domain and are grouped into four classes with SIRT6 being a member of class IV. Human SIRT6 protein is a NAD(+)-dependent histone H3 lysine-9 deacetylase that modulates telomeric chromatin (1). SIRT6 associates specifically with telomeres and SIRT6 depletion leads to telomere dysfunction with end-to-end chromosomal fusions and premature cellular senescence. SIRT6 -/- mouse cells show that SIRT6 promotes resistance to DNA damage and suppresses genomic instability in association with a role in base excision repair (2).
References:
1. Michishita, E. et al: SIRT6 is a histone H3 lysine 9 deacetylase that modulates telomeric chromatin. Nature 452: 492-496, 2008.2. Mostoslavsky, R. et al: Genomic instability and aging-like phenotype in the absence of mammalian SIRT6. Cell 124: 315-329, 2006.
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抗人球蛋白试验,又称Coombs’试验,是诊断自身免疫性溶血性贫血(AIHA)的重要依据。
正常人直接与间接试验均为阴性,Coombs’试验阴性有时并不能排除AIHA

