Although sequencing of bulk T cell samples has helped to further understanding of T-cell receptor (TCR) repertoire diversity, bulk sequencing cannot determine the pairing of specific alpha-beta receptor chains within thepopulation of T cells. Since the unique alpha-beta pairing of a TCR mediates antigen specificity, obtaining pairing information will be crucial for providing insights on antigen recognition. While sequencing of single T cells can determine the pairing on a given cell, it can be prohibitively expensive and involves complicated analyses since most methods require sequencing of a large number of cells.
Although sequencing of bulk T cell samples has helped to further understanding of T-cell receptor (TCR) repertoire diversity, bulk sequencing cannot determine the pairing of specific alpha-beta receptor chains within the population of T cells. Since the unique alpha-beta pairing of a TCR mediates antigen specificity, obtaining pairing information will be crucial for providing insights on antigen recognition. While sequencing of single T cells can determine the pairing of a given cell, it can be prohibitively expensive and involves complicated analyses since most methods require sequencing of a large number of cells.
With the SMARTer Human scTCR a/b Profiling Kit, we solve this problem by performing SMART cDNA synthesis with a set of unique indexed oligos that allow the condensing of 96 samples, which have been sorted manually or via FACS, into twelve pools for sequencing. Additionally, the approach enables the twelve pools to be further multiplexed such that all 96 samples are run in a single flow-cell lane. Much like the SMARTer Human TCR a/b Profiling Kit for bulk samples, this kit combines SMART cDNA synthesis and RACE-based PCR, followed by TCR gene-specific PCR to fully capture and amplify TCR-alpha and TCR-beta variable regions to generate Illumina-ready libraries that provide a highly sensitive approach to sequencing TCRs.
If you are looking for the software to demultiplex your sequencing data generated with this kit, please visit the SMARTer Human scTCR Demultiplexer webpage.
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鼠抗人CD3单克隆抗体针对人的CD3
CD3单克隆抗体，要看其说明书，该抗体是否识别其他生物（如大鼠）的CD3.
本试验阳性结果主要见于下列几种情况：
1.自身免疫性贫血，（IgG）型引起的溶血性贫血，本试验直接反应常呈强阳性，间接反应大多阴性，但亦可阳性。
2.药物诱发的免疫性溶血性贫血①α-甲基多巴型：直接及间接反应均阳性。②青霉素型：直接反应阳性，间接反应阴性，以上二型如以正常红细胞先与有关药物于37℃培育后再加病人血清、间接反应均为阳性。③福阿亭型：（奎宁等药物）抗体通常为IgM，偶有IgG型者，直接反应为阳性，间接反应阴性，但如用IgG抗血清做试剂则结果大部分均为阴性，但如培育时加入新鲜正常人血清（供应补体）则结果为阳性。
3.冷凝集素综合征直接反应阳性，间接反应阴性（试验需在37℃下进行）由于本病红细胞膜附着的是补体C4和C3而不是IgG或IgM，如果用抗IgG或抗IgM抗血清做试验时，则结果阴性，如以抗补体的抗血清做试验则直接反应阳性。
4.新生儿同种免疫溶血病，因Rh血型不合所致溶血病，直接及间接反应均强阳性，持续数周、换血输血后数天内可变为阴性，由于“ABO”血型不合引起的溶血病，结果常为阴性或弱阳性。
5.红细胞血型不合引起的输血反应，ABO或Rh血型不合输血，供者的红细胞被受者的血型抗体致敏，在供者被致敏的红细胞完全破坏以前，直接反应阳性，Rh阴性者如过去不曾接受过Rh阳性者的血或曾妊娠胎儿为Rh阳性者，间接反应阳性，如无上述接触，第一次输血后（Rh阳性的血），数天之内间接反应也会变为阳性。
6.其它在传染性单核细胞增多症、SLE、恶性淋巴瘤、慢性淋巴细胞白血病、癌肿、铅中毒、结节性动脉周围炎、EVan氏综合征等，病人直接反应亦可阳性，阵发性寒冷性血红蛋白尿症患者中，急性发作后用抗补体血清做试验直接反应常为阳性。展开
