The Human Mitochondrial DNA (mtDNA) Monitoring Primer Set is designed to quantify the relative number of copies of human mitochondrial DNA (mtDNA) by real-time PCR, using nuclear DNA (nDNA) content as a standard. This kit contains primer pairs for the amplification of four regions: two primer pairs for detecting mtDNA and two primer pairs for detecting nDNA. These primers will not amplify pseudogenes.
The Human Mitochondrial DNA (mtDNA) Monitoring Primer Set is designed to quantify the relative number of copies of human mitochondrial DNA (mtDNA) by real-time PCR, using nuclear DNA (nDNA) content as a standard. This kit contains primer pairs for the amplification of four regions: two primer pairs for detecting mtDNA and two primer pairs for detecting nDNA. These primers will not amplify pseudogenes. As the primers are human-specific and will not amplify DNA from mice, human mtDNA content can be monitored in induced pluripotent stem (iPS) cells or other cells cultured with feeder cells of mouse origin. This allows the Human Mitochondrial DNA (mtDNA) Monitoring Primer Set to be used in drug discovery applications where iPS cells are used as a source of differentiated liver cells, since hepatocytes have a higher ratio of mtDNA to nuclear DNA than pluripotent cells.
Using the four primer pairs in this set for real-time PCR, relative quantification of mtDNA is determined from the difference in Ct values for mtDNA and nDNA. This product provides a simple method for monitoring changes in the mtDNA copy number as mtDNA has been reported to increase during differentiation of pluripotent cells, such as iPS cells, into hepatic cells. The primer pairs in this set are compatible with Terra qPCR Direct TB Green Premix or TB Green Premix Ex Taq II (Tli RNaseH Plus).
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鼠抗人CD3单克隆抗体针对人的CD3
CD3单克隆抗体，要看其说明书，该抗体是否识别其他生物（如大鼠）的CD3.
