The human complement system is an ancient system for host defense, consisting of > 20 proteins present in plasma and cell surfaces. These proteins interact with each other to opsonize pathogens and induce a series of inflammatory responses to fight infection.C5 is the fifth component of complement, comprising alpha and beta polypeptide chains linked by a disulfide bond. C5a anaphylatoxin is derived from the alpha chain after cleavage by a convertase upon activation of C5. It is a small polypeptide consisting of 74 amino acids (11 kD). C5a itself is short-lived and cleaved rapidly into a more stable but still biologically active C5a-desArg. Therefore, measurement of C5a-desArg allows reliable quantification of the level of complement activation in the test samples. For convenience, both forms are referred to as C5a in the following manual. Human C5a shares 64% and 62% amino acid sequence identity with mouse and rat, respectively.C5a is probably the most important complement-derived proinflammatory mediator. It induces the construction of smooth muscle, increases vascular permeability, and causes histamine release. It also stimulates the chemotaxis of polymorphonuclear leukocytes towards sites of inflammation. C5a is believed to play a pivotal role in the pathogenesis of septic shock, adult respiratory distress syndrome, acute pancreatitis and the deleterious effects after myocardial infarction.The LEGEND MAX™ Human C5a ELISA kit is a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with a 96-well strip plate that is pre-coated with a mouse monoclonal anti-human C5a antibody. The Detection Antibody is a biotinylated mouse monoclonal anti-human C5/C5a antibody. This kit is specifically designed for the accurate quantitation of human C5a from serum, plasma, and other biological fluids. This kit is analytically validated with ready-to-use reagents.
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抗人球蛋白试验，又称Coombs’试验，是诊断自身免疫性溶血性贫血(AIHA)的重要依据。
正常人直接与间接试验均为阴性，Coombs’试验阴性有时并不能排除AIHA
