TheSK7antibodyisspecificforhumanCD3e,alsoknownasCD3epsilon,a20kDasubunitoftheTcellreceptorcomplex,alongwithCD3gammaandCD3delta.TheseintegralmembraneproteinchainsassemblewithadditionalchainsoftheTcellreceptor(TCR),aswellasCD3zetachain,toformtheTcellreceptor-CD3complex.Togetherwithco-receptorsCD4orCD8,thecomplexservestorecognizeantigensboundtoMHCmoleculesonantigen-presentingcells.TheseinteractionspromoteTcellreceptorsignaling(Tcellactivation),inducingcellproliferation,differentiation,productionofcytokinesoractivation-inducedcelldeath.CD3isdifferentiallyexpressedduringthymocyte-to-TcelldevelopmentandonallmatureTcells.
TheSK7antibodyisawidelyusedphenotypicMarkerforhumanTcells.ThisantibodymayinduceTcellactivationinthepresenceofmonocytes.Theantibodyhasalsobeendemonstratedtobecross-reactivewithChimpanzeeCD3.BindingofcloneSK7canbeblockedbyanalternativeAnti-HumanCD3clone,OKT3.Pleasechoosetheappropriateformatforeachapplication.
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KaneokaH,Perez-RojasG,SasasukiT,BenikeCJandEnglemanEG.1983.JImmunol.131:158-164.(invitroactivation)
HaringmanJJ,VinkenoogM,GerlagDM,SmeetsTJM,ZwindermanAHandTakPP.2005.ArthritisResTher.7(4):R862-R867.(Immunohistochemistry-frozentissue)
GovalJ-J,GreimersR,BoniverJanddeLevalL.2006.JHistochemCytochem.54(1):75-84.(Immunofluorescence-frozentissue)
WrannCD,LaueT,HubnerL,KuhlmannS,JacobsR,GoudevaLandNaveH.2012.AmJPhysiolEndocrinolMetab.302(1):E108-E116.(FlowCytometry)
SotoPC,SteinLL,Hurtado-ZiolaN,HedrickSMandVarkiA.2010.JImmunol.184(8):4185-4195.(FlowCytometry-Chimpanzee)
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抗人球蛋白试验,又称Coombs’试验,是诊断自身免疫性溶血性贫血(AIHA)的重要依据。
正常人直接与间接试验均为阴性,Coombs’试验阴性有时并不能排除AIHA

