The BC96 antibody is specific for human CD25, a 55 kDa surface protein also known as the Interleukin-2 Receptor alpha chain, or IL-2R alpha. CD25 may bind IL-2 by itself, although with low affinity and without induction of cell signaling. CD25 is also expressed within a high-affinity complex, along with the IL-2R beta chain (CD122) and the common gamma chain (CD132), to form a signaling receptor complex. Signaling via CD25 may modulate Th17 and Treg cell differentiation, and has been shown to play a role in the function of dendritic cells.
The BC96 antibody may be used as a marker for expression of CD25 during T and B cell development, on activated mature T and B cells, and for its expression at high levels on natural T regulatory cells (nTreg cells) in the thymus or periphery, and on -induced- peripheral Tregs. This antibody is cross-reactive with CD25 in several non-human species, including Baboon, Chimpanzee, Cynomolgus and Rhesus.
Willer DO, Ambagala APN, Pilon R, chan JK, Fournier J, Brooks J, Sandstrom P, and MacDonald KS. 2012. J. Virol. 86: 3626-3634. (Flow cytometry - cynomolgus)
Ruffell B, Au A, Rugo HS, Esserman LJ, Hwang ES, and Coussens LM. 2012. Proc. Natl. Acad. Sci. 109: 2796-2801. (Flow cytometry)
Mackroth MS, Malhotra I, Mungai P, Koech D, Muchiri E, and King CL. 2011. J. Immunol. 186: 2780-2791. (Flow cytometry)
Mori T, Miyamoto T, Yoshida H, Asakawa M, Kawasumi M, Kobayashi T, Morioka H, Chiba K, Toyama Y, and Yoshimura A. 2011. Int. Immunol. 23(11):701-712. (Cell sorting / negative selection - T cells)
Wan Q, Kozhaya L, ElHed A, Ramesh R, Carlson TJ, Djretic IM, Sundrud MS, and Unutmax D. 2011. J. Exp. Med. 208: 1875-1887. (Cell sorting / negative selection - T cells)
Verhoeven D, Sankaran S, Silvey M, and Dandekar S. 2008. J. Virol. 82: 4016-4027. (Flow cytometry - rhesus macaque)
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抗人球蛋白试验，又称Coombs’试验，是诊断自身免疫性溶血性贫血(AIHA)的重要依据。
正常人直接与间接试验均为阴性，Coombs’试验阴性有时并不能排除AIHA
