TheHI30antibodyreactswithhumanCD45,oneofthemostabundanthematopoieticMarkersandonethatisexpressedonallleukocytes(theLeukocyteCommonAntigen,LCA).CD45isaproteintyrosinephosphataseexistinginseveralisoforms,eachbeinggeneratedandexpressedincell-specificpatterns.Withitsbroadcelldistribution,CD45iscriticalformanyleukocytefunctions,regulatingsignaltransductionandcellactivationassociatedwiththeTcellreceptor,Bcellreceptor,andIL-2receptor.OtherformsofCD45,withrestrictedcellularexpression,includeCD45R(B220),CD45RA,CD45RB,CD45ROandothers.
TheHI30antibodyiswidelyusedasamarkerforhumanCD45expressiononTcells,Bcells,monocytes,macrophages,andNKcells.
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抗人球蛋白试验,又称Coombs’试验,是诊断自身免疫性溶血性贫血(AIHA)的重要依据。
正常人直接与间接试验均为阴性,Coombs’试验阴性有时并不能排除AIHA

