Tested Species Reactivity:HumanProduct Format:LyophilizedApplication:CAAdditional Information:Description: CD30 ligand, also known as CD153 and TNFSF8, is a Type II glycoprotein and a member of the TNF superfamily. It is expressed on the surface of activated B cell subsets, T cells and monocytes. In the presence of CD30, CD30L is cleaved and the soluble form binds to CD30+ cells which can induce apoptosis in some cases. CD30L/CD30 interactions also generate signaling pathways leading to either cell death or proliferation. Studies suggest a role for CD30L/CD30 signaling in Th1, Th2 and Th17 responses, as well as in certain diseases such as allergic inflammation, rheumatoid arthritis and Hodgkin"s lymphoma.:::Bioactivity: Using a concentration range of 10.0-25.0 ng/ml, the ability to stimulate human IL-8 production in human PBMC is determined. Results may vary due to differences between PBMC donors.:::Endotoxin Level: Endotoxin level is <0.1 ng/ug="" of="" protein="">0.1><1>1>Reconstitution and Storage:-20CPurity:98%Protein Sequence:HHHHHHHHPS PGGSGGQRTD SIPNSPDNVP LKGGNCSEDL LCILKRAPFK KSWAYLQVAK HLNKTKLSWN KDGILHGVRY QDGNLVIQFP GLYFIICQLQ FLVQCPNNSV DLKLELLINK HIKKQALVTV CESGMQTKHV YQNLSQFLLD YLQVNTTISV NVDTFQYIDT STFPLENVLS IFLYSNSDSource:CHO cellsTarget Reference:Kennedy MK, Willis CR and Armitage RJ. 2006. Immunology. 118(2): 143-152.
Oflazoglu E, Grewal IS and Gerber H. 2009. Adv Exp Med Biol. 647: 174-185
Tinazzi E, Barbieri A, Rigo A, Patuzzo G, Beri R, Gerli R, Argentino G, Puccetti A and Lunardi C. 2014. Immunol Lett. 161(2): 236-240.
Bengtsson A. 2001. Allergy. 56(7): 593-603.
Sun X, Yamada H, Shibata K, Muta H, Tani K, Podack ER and Yoshikai Y. 2010. J Immunol. 185(4): 2222-2230.
Wiley SR, Goodwin RG and Smith CA. 1996. J Immunol. 157(8): 3635-3639.
Datasheets/Manuals:Printable datasheet for OPTA00923Gene Symbol:TNFSF8Official Gene Full Name:TNF superfamily member 8Alias Symbols:CD153, CD30L, CD30LG, TNLG3ANCBI Gene Id:944Protein Name:tumor necrosis factor ligand superfamily member 8Description of Target:The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF8/CD30, which is a cell surface antigen and a marker for Hodgkin lymphoma and related hematologic malignancies. The engagement of this cytokine expressed on B cell surface plays an inhibitory role in modulating Ig class switch. This cytokine was shown to enhance cell proliferation of some lymphoma cell lines, while to induce cell death and reduce cell proliferation of other lymphoma cell lines. The pleiotropic biologic activities of this cytokine on different CD30+ lymphoma cell lines may play a pathophysiologic role in Hodgkin's and some non-Hodgkin's lymphomas. Two transcript variants encoding different isoforms have been found for this gene.Molecular Weight:21.3 kDaTissue Tool:Find tissues and cell lines supported by DNA array analysis to express TNFSF8. RNA Seq:Find tissues and cell lines supported by RNA-seq analysis to express TNFSF8.