Tested Species Reactivity:HumanProduct Format:LyophilizedApplication:CAAdditional Information:Description: IL-4R alpha is a Type I transmembrane protein belonging to the class I cytokine receptor family and is a component of two different receptor complexes. When associated with the common gamma chain, which is required for signaling, it binds IL-4 with higher affinity. When complexed with IL-13R alpha I, interaction with both IL-4 and IL-13 is enabled. Through JAK/STAT signal transduction pathways, cell-specific responses including Th2 differentiation, mucosal immunity, allergic inflammation and IgE production result. The soluble form of IL-4R alpha, generated through proteolysis in the human, retains biologic activity and is able to antagonize IL-4.:::Bioactivity: The expected ED50 of <= 5.0="" ng/ml,="" corresponding="" to="" a="" specific="" activity="" of="">= 2 x 105 units/mg, is measured by determining the ability to inhibit the IL-4 dependent proliferation of human TF-1 cells while in the presence of 0.5 ng/ml I=>:::Endotoxin Level: Endotoxin level is <0.1 ng/ug="" of="" protein="">0.1><1>1>Reconstitution and Storage:-20CPurity:98%Protein Sequence:GNMKVLQEPT CVSDYMSIST CEWKMNGPTN CSTELRLLYQ LVFLLSEAHT CIPENNGGAG CVCHLLMDDV VSADNYTLDL WAGQQLLWKG SFKPSEHVKP RAPGNLTVHT NVSDTLLLTW SNPYPPDNYL YNHLTYAVNI WSENDPADFR IYNVTYLEPS LRIAASTLKS GISYRARVRA WAQCYNTTWS EWSPSTKWHN SYREPFEQHSource:HEK293 cellsTarget Reference:Maliszewski CR, Sato TA, Davison B, Jacobs CA, Finkelman FD and Fanslow WC. 1994. Proc Soc Exp Biol Med. 206(3): 233-7.
Kruse S, Forster J, Kuehr J and Deichmann KA. 1999. Int Immunol. 11(12): 1965-1970.
Kondo M, Takeshita T, Ishii N, Nakamura M, Watanabe S, Arai K and Sugamura K. 1993. Science. 262(5141): 1874-1877.
Wills-Karp M and Finkelman FD. 2008. Sci Signal. 1(51): pe55.
Jung T, Schrader N, Hellwig M, Enssle KH and Neumann C. 1999. Int Arch Allergy Immunol. 199(1): 23-30.
Datasheets/Manuals:Printable datasheet for OPTA00928Gene Symbol:IL4ROfficial Gene Full Name:interleukin 4 receptorAlias Symbols:CD124, IL4RA, IL-4RANCBI Gene Id:3566Protein Name:interleukin-4 receptor subunit alphaDescription of Target:This gene encodes the alpha chain of the interleukin-4 receptor, a type I transmembrane protein that can bind interleukin 4 and interleukin 13 to regulate IgE production. The encoded protein also can bind interleukin 4 to promote differentiation of Th2 cells. A soluble form of the encoded protein can be produced by proteolysis of the membrane-bound protein, and this soluble form can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells. Allelic variations in this gene have been associated with atopy, a condition that can manifest itself as allergic rhinitis, sinusitus, asthma, or eczema. Polymorphisms in this gene are also associated with resistance to human immunodeficiency virus type-1 infection. Alternate splicing results in multiple transcript variants.Tissue Tool:Find tissues and cell lines supported by DNA array analysis to express IL4R. RNA Seq:Find tissues and cell lines supported by RNA-seq analysis to express IL4R.