Tested Species Reactivity:HumanPredicted Species Reactivity:MouseProduct Format:LyophilizedApplication:CAAdditional Information:Description: IL-13, produced primarily by Th2 cells and to a lesser extent by mast and NK cells, is an immunoregulatory cytokine. Its various functions include induction of matrix metalloproteinases, enhancement of IgE secretion from activated B cells and B cell proliferation. Additionally, IL-13 plays a role in the inhibition of the expression of inflammatory cytokines and modulates resistance to various parasites. IL-13 is also secreted by eosinophils and in the lung is essential in regulating eosinophilic inflammation, mucus secretion, and airway hyperresponsiveness. Its function as a central mediator of allergen-induced asthma is well documented. Single nucleotide polymorphisms result in variants associated with specific asthmatic or allergic phenotypes, and certain variants will show enhanced functional activity as compared to wild-type IL-13.:::Bioactivity: Compared to wild type IL-13, this variant shows a two fold increase in bioactivity both in vitro and in vivo. Dose dependent STAT6 activation and IL-13 dependent gene induction in transfected A201.1 cells were used to demonstrate this difference in vitro.:::Endotoxin Level: Endotoxin level is <0.1 ng/ug="" of="" protein="">0.1><1>1>Reconstitution and Storage:-20CPurity:98%Protein Sequence:MSPGPVPPST ALRELIEELV NITQNQKAPL CNGSMVWSIN LTAGMYCAAL ESLINVSGCS AIEKTQRMLS GFCPHKVSAG QFSSLHVRDT KIEVAQFVKD LLLHLKKLFR EGQFNSource:E. coliTarget Reference:McKenzie AN, Culpepper JA, de Waal Malefyt R, Brière F, Punnonen J, Aversa G, Sato A, Dang W, Cocks BG and Menon S. 1993. Proc Natl Acad Sci USA. 90(8): 3735-3739.
Ying S1, Meng Q, Barata LT, Robinson DS, Durham SR, Kay AB. 1997. J Immunol. 158(10): 5050-5057.
Wynn TA. 2003. Annu Rev Immunol. 21: 425-456.
Vercelli D. 2002. Curr Opin Allergy Clin Immunol. 2(5): 389-393.
Minty A, Chalon P, Derocq JM, Dumont X, Guillemot JC, Kaghad M, Labit C, Leplatois P, Liauzun P and Miloux B 1993 Nature 362(6417): 248-50.
Datasheets/Manuals:Printable datasheet for OPTA00930Gene Symbol:IL13Official Gene Full Name:interleukin 13Alias Symbols:P600, IL-13NCBI Gene Id:3596Protein Name:interleukin-13Description of Target:This gene encodes an immunoregulatory cytokine produced primarily by activated Th2 cells. This cytokine is involved in several stages of B-cell maturation and differentiation. It up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. This cytokine down-regulates macrophage activity, thereby inhibits the production of pro-inflammatory cytokines and chemokines. This cytokine is found to be critical to the pathogenesis of allergen-induced asthma but operates through mechanisms independent of IgE and eosinophils. This gene, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL4.Molecular Weight:12.6 kDaTissue Tool:Find tissues and cell lines supported by DNA array analysis to express IL13. RNA Seq:Find tissues and cell lines supported by RNA-seq analysis to express IL13.