Tested Species Reactivity:HumanProduct Format:LyophilizedApplication:CAAdditional Information:Description: Angiopoietin-1 (ANG-1, ANGPT1) is a secreted glycoprotein belonging to the angiopoietin family of growth factors that play a critical role in the regulation of angiogenesis and vascular development. Angiopoietin-1 is a ligand for receptor tyrosine kinase Tie-2, and ligation to Tie-2 on vascular endothelial cells promotes blood vessel development and stabilization of mature blood vessels. The process is regulated by Angiopoietin-2, a natural antagonist for Tie-2, and by Tie-1, an inhibitory co-receptor.:::Bioactivity: A dose-dependent proliferation assay with human umbilical vein endothelial cells (HUVECs) is used to determine activity.:::Endotoxin Level: Endotoxin level is <0.1 ng/ug="" of="" protein="">0.1><1>1>Reconstitution and Storage:-20CPurity:95%Protein Sequence:SNQRRSPENS GRRYNRIQHG QCAYTFILPE HDGNCRESTT DQYNTNALQR DAPHVEPDFS SQKLQHLEHV MENYTQWLQK LENYIVENMK SEMAQIQQNA VQNHTATMLE IGTSLLSQTA EQTRKLTDVE TQVLNQTSRL EIQLLENSLS TYKLEKQLLQ QTNEILKIHE KNSLLEHKIL EMEGKHKEEL DTLKEEKENL QGLVTRQTYI IQELEKQLNR ATTNNSVLQK QQSource:HeLa cellsTarget Reference:Tsigkos S, Koutsilieris M and Papapetropoulos A. 2003. Expert Opin Investig Drugs. 12(6): 933-941.
Davis S, Aldrich TH, Jones PF, Acheson A, Compton DL, Jain V, Ryan TE, Bruno J, Radziejewski C, Maisonpierre PC, et. al. 1996. Cell. 87(7): 1161-1169.
Sato A, Iwama A, Takakura N, Nishio H, Yancopoulos GD and Suda T. 1998. Int Immunol. 10(8):1217-1227.
Singh H, Tahir TA, Alawo DO, Issa E and Brindle NP. 2011. Biochem Soc Trans. 39(6): 1592-1596.
Koh GY. 2013. Trends Mol Med. 19(1): 31-39.
Datasheets/Manuals:Printable datasheet for OPTA00945Gene Symbol:ANGPT1Official Gene Full Name:angiopoietin 1Alias Symbols:AGP1, AGPT, ANG1NCBI Gene Id:284Protein Name:angiopoietin-1Description of Target:This gene encodes a secreted glycoprotein that belongs to the angiopoietin family. Members of this family play important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme and inhibits endothelial permeability. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart. Alternative splicing results in multiple transcript variants encoding distinct isoforms.Molecular Weight:56.3 kDaTissue Tool:Find tissues and cell lines supported by DNA array analysis to express ANGPT1. RNA Seq:Find tissues and cell lines supported by RNA-seq analysis to express ANGPT1.