Tested Species Reactivity:HumanPredicted Species Reactivity:MouseProduct Format:LyophilizedApplication:CAAdditional Information:Description: CD14, expressed mainly by monocytes and macrophages, is generally present as a membrane-bound surface glycoprotein. It serves as a homing receptor for lipopolysaccharide (LPS) and is a pattern recognition receptor for a variety of microbial-derived ligands. CD14 is not a signaling protein and forms a receptor complex with toll-like receptor 4 (TLR4) to transduce CD14-LPS signals. CD14 is also present as a soluble format in serum and other bodily fluids (sCD14). This soluble format can be shed from the cell surface and may play a role in inhibiting LPS-mediated responses in extracellular spaces.:::Bioactivity: Using a concentration range of 20 - 200 ng/ul, dose dependent activation of NF-kB in a RAW264 cell line based reporter system is measured. In the presence of 0.25 - 1.0 ng/ul of bacterial LPS enhances the activation assay.:::Endotoxin Level: Endotoxin level is <0.1 ng/ug="" of="" protein="">0.1><1>1>Reconstitution and Storage:-20CPurity:95%Protein Sequence:TTPEPCELDD EDFRCVCNFS EPQPDWSEAF QCVSAVEVEI HAGGLNLEPF LKRVDADADP RQYADTVKAL RVRRLTVGAA QVPAQLLVGA LRVLAYSRLK ELTLEDLKIT GTMPPLPLEA TGLALSSLRL RNVSWATGRS WLAELQQWLK PGLKVLSIAQ AHSPAFSCEQ VRAFPALTSL DLSDNPGLGE RGLMAALCPH KFPAIQNLAL RNTGMETPTG VCAALAAAGV QPSource:HEK293 cellsTarget Reference:Wright SD, Ramos RA, Tobias PS, Ulevitch RJ and Mathison JC. 1990. Science. 249(4975): 1431-1433.
Zanoni I, Ostuni R, Marek LR, Barresi S, Barbalat R, Barton GM, Granucci F and Kagan JC. 2011. Cell. 147(4): 868-880.
Jersmann. 2005. Immunol Cell Biol. 83(5): 462-467.
Pugin J, Schurer-Maly CC, Leturcq D, Moriarty A, Ulevitch RJ and Tobias PS. 1993. Proc Natl Acad Sci USA. 90(7): 2744-2748.
Stelter F. 2000. Chem Immunol. 74: 25-41.
Datasheets/Manuals:Printable datasheet for OPTA00951Gene Symbol:CD14Official Gene Full Name:CD14 moleculeAlias Symbols:soluble CD14, Monocyte Differentiation Antigen CD14NCBI Gene Id:929Protein Name:monocyte differentiation antigen CD14Description of Target:The protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide. Alternative splicing results in multiple transcript variants encoding the same protein.Molecular Weight:35.6 kDaTissue Tool:Find tissues and cell lines supported by DNA array analysis to express CD14. RNA Seq:Find tissues and cell lines supported by RNA-seq analysis to express CD14.