Tested Species Reactivity:HumanPredicted Species Reactivity:Mouse, Human, ChickenProduct Format:LyophilizedApplication:CAAdditional Information:Description: CYR61, also known as CCN1, is a matricellular signaling molecule that binds to a number of cell surface proteins thereby modulating a variety of signaling pathways. CYR61 is expressed in a variety of cells and is capable of regulating cell adhesion, migration, proliferation, differentiation, apoptosis, and senescence through interaction with cell surface integrin receptors and heparan sulfate proteoglycans. CYR61 performs critical functions during embryonic development, and in adulthood CYR61 plays important roles in inflammation and tissue repair. It is associated with diseases related to chronic inflammation. Deregulated expression and signaling of CYR61 is associated with a variety of tumors exhibiting both tumorigenic and tumor suppressor properties. Full-length CYR61 protein contains 381 amino acids with an N-terminal secretory signal peptide followed by four structurally distinct domains (modules); the IGF binding protein (IGFBP) domain, the von Willebrand Factor C (VWFC) domain, the Thrombospondin type-I (TSP type-1) domain, and a C-terminal cysteine knot-like domain (CTCK).:::Bioactivity: Determined by the dose-dependent stimulation of the proliferation of murine 3T3 cells. The expected ED50 for this effect is 2.0-3.0 ug/ml.:::Endotoxin Level: Endotoxin level is <0.1 ng/ug="" of="" protein="">0.1><1>1>Reconstitution and Storage:-20CPurity:95%Protein Sequence:TCPAACHCPL EAPKCAPGVG LVRDGCGCCK VCAKQLNEDC SKTQPCDHTK GLECNFGASS TALKGICRAQ SEGRPCEYNS RIYQNGESFQ PNCKHQCTCI DGAVGCIPLC PQELSLPNLG CPNPRLVKVT GQCCEEWVCD EDSIKDPMED QDGLLGKELG FDASEVELTR NNELIAVGKG SSLKRLPVFG MEPRILYNPL QGQKCIVQTT SWSQCSKTCG TGISTRVTND NPSource:E.coliTarget Reference:Francischetti IM, Kotsyfakis M, Andersen JF and Lukszo J. 2010. PLoS One. 5(2):e9356.
Lorda-Diez CI, Montero JA, Diaz-Mendoza MJ, Garcia-Porrero JA and Hurle JM. 2011. PLoS One. 6(9):e24546.
Lin J, Zhou Z, Huo R, Xiao L, Ouyang G, Wang L, Sun Y, Shen B, Li D and Li N. 2012. J Immunol. 188(11):5776-84.
Schlage P, Kockmann T, Sabino F, Kizhakkedathu JN, Auf dem Keller and U. 2015. Mol Cell Proteomics. 4(12):3234-46.
Yu Y, Prassas I, Dimitromanolakis A and Diamandis EP. 2015. J Biol Chem. 290(29):17762-75.
Datasheets/Manuals:Printable datasheet for OPTA00953Gene Symbol:CCN1Official Gene Full Name:cellular communication network factor 1Alias Symbols:GIG1, CYR61, IGFBP10NCBI Gene Id:3491Protein Name:CCN family member 1Description of Target:The secreted protein encoded by this gene is growth factor-inducible and promotes the adhesion of endothelial cells. The encoded protein interacts with several integrins and with heparan sulfate proteoglycan. This protein also plays a role in cell proliferation, differentiation, angiogenesis, apoptosis, and extracellular matrix formation.Molecular Weight:39.5 kDaTissue Tool:Find tissues and cell lines supported by DNA array analysis to express CCN1. RNA Seq:Find tissues and cell lines supported by RNA-seq analysis to express CCN1.