

Thisproductisfreezedried.Allwatermoleculeshavebeenremoved.

ThisantibodyisshippedwithitsantigenFREEofcharge!
- Peptide(C)DDYGRPGIEKFREE,correspondingtoaminoacidresidues216-229ofhumanCaSR(Accession P41180). N-terminus,extracellular.
- WesternblotanalysisofColo205(lanes1and3),ratliver(lanes2and4)andmousekidney(lanes5and6)lysates:1,2,5. Anti-CalciumSensingReceptor(extracellular)Antibody(#ACR-004),(1:200).
3,4,6.Anti-CalciumSensingReceptor(extracellular)Antibody,preincubatedwiththecontrolpeptideantigen.
- ExpressionofCaSRinratbrainImmunohistochemicalstainingofrathippocampalCA1regionusing Anti-CalciumSensingReceptor(extracellular) Antibody(#ACR-004).A.CaSRstainingappearsinthesomaandappicaldendritesofCA1pyramidalneurons(arrows).B.NuclearstainingusingDAPIasthecounterstain.C.MergeimageofAandBpannels
- CellsurfacedetectionofCaSRinliveJurkat(humanacuteTcellleukemia)cells:___ Unstainedcells.
___ Cells+ Anti-CalciumSensing Receptor(extracellular) Antibody(#ACR-004),(10µg/5x105 cells).
- Expressionof CaSRinratC6cellsCellsurfacedetectionof CaSRinliveintactratgliomaC6cells.A.Extracellularstainingofcellsusing Anti-CalciumSensing Receptor(extracellular) Antibody(#ACR-004),(1:50),followedbyAlexa-555-conjugatedgoat-anti-rabbitsecondaryantibody.B.NuclearfluorescencestainingofcellsusingHoechst33342.C.MergedimagesofpanelsAandB.
- 1.IUPHARRECEPTORDATABASE.
- 2.Racz,G.Z.etal.(2002)Gut51,705.
- 3.Canaff,L.andHendy,G.N.(2002)J.Biol.Chem.277,30337.
- 4.Chen,R.A.andGoodman,W.G.(2004)Am.J.Physiol.RenalPhysiol.286,F1005
- 5.Chattopadhyay,N.(2006)Am.J.Physiol.Endocrinol.Metab.290,E761.
TheCalcium-sensingreceptor(CaSR)isamemberofsubfamilyCoftheG-protein-coupledreceptor(GPCR)superfamily.Asitsprimaryrole,theCaSRisakeyplayerincalciumhomeostasisandisexpressedintissuesinvolvedincalciummetabolismsuchasparathyroidcellsandthekidneys.1,2
TheexpressionofCaSRwasalsodescribedinothercelltypesandtissues,suchasneurons,keratinocytes,andthepancreas.However,theroleofCaSRinthesecellsremainstobeestablished.1,2 FollowingtheidentificationoffunctionalvitaminDresponseelementsintheCaSRgene,itwassuggestedthatvitaminDmightregulatetheexpressionofCaSR.3
CaSRactivationisfollowedbyarapid,transientincreaseinthecytosoliccalciumconcentration,resultingfrommobilizationofcalciumfromthapsigargin-sensitiveintracellularstores,aswellasanincreasedcalciuminfluxthroughvoltage-insensitivecalciumchannelslocatedatthecellmembrane.4
CaSRregulatescellularprocessessuchasproliferation,apoptosis,anddifferentiationunderbothnormalandpathologicconditions.5
RecentstudiesdemonstratedtheexpressioninhumancolonepitheliumofCaSR,whichregulatesproliferationanddifferentiation.Incoloncarcinomas,lowerlevelsofexpressionwerefoundinthecanceroustissuecomparedtonormalcolontissue.
Anti-CalciumSensingReceptor(extracellular)Antibody(#ACR-004)isahighlyspecificantibodydirectedagainstanepitopeofthehumanCaSR.Theantibodycanbeusedinwesternblot,immunohistochemistry,indirectflowcytometry,andimmunocytochemistry,andlivecellimagingapplications.IthasbeendesignedtorecognizeCaSRfromhuman,rat,andmousesamples.
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不知道能不能直接买到大鼠抗豚鼠血管IgG的ELISA试剂盒。
如果买不到这种试剂盒,用空白板的话(就是反应孔中没有抗原或者抗体包被),把自制的豚鼠血管抗原加到反应孔中进行孵育,能不能使抗原附着在孔壁上?这样的话就可以按照elisa的操作步骤进行大鼠血清检测了。
目前的设想是自制抗原,粉碎豚鼠的血管,制成悬液,通过反复多次的冻融,离心后收集上清液作为抗原(即豚鼠血管抗原)。
然后将抗原加入到elisa的反应孔中(这种是特制的空白板,就是反应孔中没有抗原或抗体包被)进行孵育,使豚鼠血管抗原附着在孔壁上,就是让反应孔充当固相载体,形成固相抗原。倒掉多余的抗原。
再加入待检测的大鼠血清,这样血清中的特异性抗豚鼠血管IgG就可以跟固相的抗原结合,形成固相抗原抗体复合物。
加入酶标的兔抗鼠或者羊抗鼠IgG,形成酶标的抗原抗体复合物。
然后就是一些显色步骤。
现在关键的问题就是抗原加入空白板,能不能形成固相抗原,如果不能跟孔壁附着的话,在后面洗涤的过程中就被洗掉了,那就没办法完成检测了。
请各位大侠给指条明路啊!!!小弟在此多谢了!!!
多谢!

