

Thisproductisfreezedried.Allwatermoleculeshavebeenremoved.

ThisantibodyisshippedwithitsantigenFREEofcharge!
- Peptide(C)KIRKNTWASHSSK,correspondingtoaminoacidresidues212-224oftheratAngiotensin-(1-7)Masreceptor(AccessionP12526).3rd intracellular loop.
- Westernblotanalysisofratbrainlysate(lanes1and3)andmousebrainmembranes(lanes2and4):1,2.Guineapig Anti-Angiotensin-(1-7)MasReceptor Antibody(#AGP-099),(1:600).
3,4.GuineapigAnti-Angiotensin-(1-7)MasReceptorAntibody,preincubatedwiththenegativecontrolantigen.
- 1.Santos,R.A.etal.(2003)Proc.Natl.Acad.Sci.U.S.A.100,8258.
- 2.Alenina,N.etal.(2008)Exp.Physiol.93,528.
- 3.Santos,R.A.etal.(2008)Exp.Physiol.93,519.
TheAngiotensin-(1-7)MasreceptoristherecentlyidentifiedreceptoroftheBIOLOGicallyactiveheptapeptideangiotensin-(1-7).1
Angiotensin(Ang)-(1-7)isametaboliteofthewellknownpeptidehormoneAngiotensin(Ang)II,akeycomponentoftherenin-angiotensinsystem(RAS)thathasacentralroleincardiovascularhomeostasis.
ConsiderablyinterestinAng-(1-7)anditsreceptorarousedinthelastfewyearssinceitbecameapparentthatitcancounterbalancemostofAngIIeffects.ThusAng-(1-7)hasvasodilatorandhypotensiveeffectsaswellasantiarrhythmicandcardioprotectiveroles.2,3
TheAng-(1-7)Masreceptorbelongstothe7-transmembranedomain,Gprotein-coupledreceptor(GPCR)superfamilyandwasoriginallydescribedasaprotooncogene.
SignalingviatheAng-(1-7)Masreceptorisstillpoorlyelucidatedhowever,evidenceindicatesthatthereceptoriscoupledtoaGq/11 proteinthatactivatesphospholipaseC(PLC).2,3
TheAng-(1-7)Masreceptorisexpressedinseveralorgansincludingheart,kidney,bloodvessels,testisandbrain.StudieswithAng-(1-7)Masreceptorknockoutmicehavedemonstratedthekeyroleofthisreceptorincardiovascularregulationaswellasintheregulationoflearningandmemory.2
AlomoneLabsispleasedtoofferahighlyspecificantibodydirectedagainstanepitopeoftheratprotein.GuineapigAnti-Angiotensin-(1-7)MasReceptorAntibody(#AGP-099)raisedinguineapigcanbeusedinwesternblotanalysis.IthasbeendesignedtorecognizeAng-(1-7)Masreceptorfromrat,mouseandhumansamples.TheantigenusedtoimmunizeguineapigsisthesameasAnti-Angiotensin-(1-7)MasReceptorAntibody(#AAR-013)raisedinrabbit.Ourlineofguineapigantibodiesenablesmoreflexibilitywithourproductssuchasimmuno-colocalizationstudies,immunoprecipitation,etc.
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不知道能不能直接买到大鼠抗豚鼠血管IgG的ELISA试剂盒。
如果买不到这种试剂盒,用空白板的话(就是反应孔中没有抗原或者抗体包被),把自制的豚鼠血管抗原加到反应孔中进行孵育,能不能使抗原附着在孔壁上?这样的话就可以按照elisa的操作步骤进行大鼠血清检测了。
目前的设想是自制抗原,粉碎豚鼠的血管,制成悬液,通过反复多次的冻融,离心后收集上清液作为抗原(即豚鼠血管抗原)。
然后将抗原加入到elisa的反应孔中(这种是特制的空白板,就是反应孔中没有抗原或抗体包被)进行孵育,使豚鼠血管抗原附着在孔壁上,就是让反应孔充当固相载体,形成固相抗原。倒掉多余的抗原。
再加入待检测的大鼠血清,这样血清中的特异性抗豚鼠血管IgG就可以跟固相的抗原结合,形成固相抗原抗体复合物。
加入酶标的兔抗鼠或者羊抗鼠IgG,形成酶标的抗原抗体复合物。
然后就是一些显色步骤。
现在关键的问题就是抗原加入空白板,能不能形成固相抗原,如果不能跟孔壁附着的话,在后面洗涤的过程中就被洗掉了,那就没办法完成检测了。
请各位大侠给指条明路啊!!!小弟在此多谢了!!!
多谢!

