Recombinant Human His6-UbcH6/UBE2E1 Protein, CF Summary
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
E2-630
| Formulation | X mg/ml (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: UbcH6/UBE2E1
Ubiquitin-conjugating Enzyme H6 (UbcH6), also known as Ubiquitin-conjugating Enzyme E2E 1 (UBE2E1), is a ubiquitously expressed protein with a predicted molecular weight of 21 kDa. Human UbcH6/UBE2E1 shares 99% and 100% amino acid sequence identity with mouse and rat UBE2E1, respectively. UbcH6/UBE2E1 mediates the selective degradation of short-lived and abnormal proteins (1). UbcH6/UBE2E1 catalytic activity is modulated following the addition of the Ubiquitin-like protein ISG15 to Lys136 (2). In the nucleus, the N-terminal fragment of UbcH6/UBE2E1 interacts with the Ubiquitin ligase (E3) TRIM21 to mediate substrate ubiquitination (3,4). UbcH6/UBE2E1 also interacts with the viral E3, ICP0, during HSV-1 infection (5). In addition, UbcH6/UBE2E1-dependent protein ubiquitination is implicated in polyglutamine tract disorders such as ataxia (6,7).
- Nuber, U. et al. (1996) J. Biol. Chem. 271: 2795.
- Takeuchi, T. et al. (2005) J. Biochem. 138:711.
- Espinosa, A. et al. (2008) Exp. Cell Res. 314:3605.
- Espinosa, A. et al. (2011) J. Biol. Chem. 286:36478.
- Vanni, E. et al. (2012) J. Virol. 86:6323.
- Hong, S. et al. (2008) Biochem. Biophys. Res. Commun. 372:256.
- Lee, S. et al. (2008) Biochem. Biophys. Res. Commun. 372:735.
- Ito K., et al. (2001) Eur J. Biochem. 268: 2725-2732
- Nuber U., et al. (1996) J. Biol. Chem. 271: 2795-800
- Plafker S.M., et al. (2004) J. Cell Biol. 167: 649-659
- Rajendra R., et al. (2004) J. Biol. Chem. 279: 36440-36444
- Vichi A., et al. (2005) Proc. Natl. Acad. Sci. 102: 1945-1950
- Wang Z., et al. (2005) J. Virol. 79: 8764-8772
FAQs
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多谢!
不知道能不能直接买到大鼠抗豚鼠血管IgG的ELISA试剂盒。
如果买不到这种试剂盒,用空白板的话(就是反应孔中没有抗原或者抗体包被),把自制的豚鼠血管抗原加到反应孔中进行孵育,能不能使抗原附着在孔壁上?这样的话就可以按照elisa的操作步骤进行大鼠血清检测了。
目前的设想是自制抗原,粉碎豚鼠的血管,制成悬液,通过反复多次的冻融,离心后收集上清液作为抗原(即豚鼠血管抗原)。
然后将抗原加入到elisa的反应孔中(这种是特制的空白板,就是反应孔中没有抗原或抗体包被)进行孵育,使豚鼠血管抗原附着在孔壁上,就是让反应孔充当固相载体,形成固相抗原。倒掉多余的抗原。
再加入待检测的大鼠血清,这样血清中的特异性抗豚鼠血管IgG就可以跟固相的抗原结合,形成固相抗原抗体复合物。
加入酶标的兔抗鼠或者羊抗鼠IgG,形成酶标的抗原抗体复合物。
然后就是一些显色步骤。
现在关键的问题就是抗原加入空白板,能不能形成固相抗原,如果不能跟孔壁附着的话,在后面洗涤的过程中就被洗掉了,那就没办法完成检测了。
请各位大侠给指条明路啊!!!小弟在此多谢了!!!

