

Thisproductisfreezedried.Allwatermoleculeshavebeenremoved.

ThisantibodyisshippedwithitsantigenFREEofcharge!
- Peptide(C)EAMNHDLELGHTHG,correspondingtoaminoacidresidues252-265ofhumanCALHM1(Accession Q8IU99).Intracellular,C-terminus.
- Westernblotanalysisofratbrain(lanes1and4),mousebrain(lanes2and5),U-87MG(lanes3and6)andC6(lanes4and8)lysates:1.Anti-CALHM1Antibody (#ACC-101),(1:200).
2.Anti-CALHM1Antibody,preincubatedwiththenegativecontrolantigen.
- Expressionof CALHM1inrathippocampusImmunohistochemicalstainingofrathippocampususingAnti-CALHM1Antibody (#ACC-101).A.Stainingof CALHM1appearsonlyinthehippocampalCA1pyramidallayer(red).B.NuclearstainingusingDAPIasthecounterstain(blue).C.MergeofAandB.
- 1.Green,K.N.andLaFerla,F.M.(2008)Neuron59,190.
- 2.Koppel,J.etal.(2011)Mol.Med.17,974.
- 3.Dreses-Werringloer,U.etal.(2008)Cell133,1149.
AmisbalanceinCa2+ homeostasisseemstoberelatedtothedevelopmentofAlzheimer’sdisease(AD).Indeed,neuronalCa2+ balancemayaffectthelevelsofproteinsassociatedwithAD,suchasamyloid-beta(Ab)andtau1.
Calciumhomeostasismodulator1(CALHM1)isacerebralCa2+ channelcomponentresponsIBLeforcontrollingintracellularCa2+ levelsandAbmetabolism2,3.CALHM1isathreetransmembraneglycoprotein,mostlylocalizedtotheendoplasmicreticulumalthoughitcanbedetectedattheplasmamembrane.AfunctionalchannelisformedbymultiplesubunitswhichhassomestructuralsimilaritiestotheNMDAreceptor.Itisexpressedinallbrainregions1.
Inculturedcells,overexpressionofCALHM1increasesintracellularCa2+ levelsandreducesAbaccumulation,therebyreinforcingitsimplicationinthedevelopmentofAD.Indeed,apolymorphisminthegenewasfoundtoaffecttheonsetofAD2.
Anti-CALHM1Antibody(#ACC-101)isahighlyspecificantibodydirectedagainstanepitopeofthehumancalciumhomeostasismodulatorprotein1.Theantibodycanbeusedinwesternblotandimmunohistochemistryapplications.IthasbeendesignedtorecognizeCALHM1fromrat,mouseandhumansamples.
ebiomall.com






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不知道能不能直接买到大鼠抗豚鼠血管IgG的ELISA试剂盒。
如果买不到这种试剂盒,用空白板的话(就是反应孔中没有抗原或者抗体包被),把自制的豚鼠血管抗原加到反应孔中进行孵育,能不能使抗原附着在孔壁上?这样的话就可以按照elisa的操作步骤进行大鼠血清检测了。
目前的设想是自制抗原,粉碎豚鼠的血管,制成悬液,通过反复多次的冻融,离心后收集上清液作为抗原(即豚鼠血管抗原)。
然后将抗原加入到elisa的反应孔中(这种是特制的空白板,就是反应孔中没有抗原或抗体包被)进行孵育,使豚鼠血管抗原附着在孔壁上,就是让反应孔充当固相载体,形成固相抗原。倒掉多余的抗原。
再加入待检测的大鼠血清,这样血清中的特异性抗豚鼠血管IgG就可以跟固相的抗原结合,形成固相抗原抗体复合物。
加入酶标的兔抗鼠或者羊抗鼠IgG,形成酶标的抗原抗体复合物。
然后就是一些显色步骤。
现在关键的问题就是抗原加入空白板,能不能形成固相抗原,如果不能跟孔壁附着的话,在后面洗涤的过程中就被洗掉了,那就没办法完成检测了。
请各位大侠给指条明路啊!!!小弟在此多谢了!!!
多谢!

