Thisproductisfreezedried.Allwatermoleculeshavebeenremoved.
ThisantibodyisshippedwithitsantigenFREEofcharge!
- GSTfusionproteinwiththesequenceTLSKSEYMVIEEGGMNHSAFPQTPFKTGNSTATCTTNNNPNSCVNIKKIFTDV,correspondingtoaminoacidresidues523-575ofhumanKV1.3(Accession P22001).Intracellular,C-terminus.
- Westernblotanalysis ofratbrainmembranes:1. Anti-KV1.3 (KCNA3) Antibody(#APC-002),(1:200).
2.Anti-KV1.3(KCNA3)Antibody,preincubatedwiththenegativecontrolantigen.HumanCD3+cells(Szigligeti,P. etal. (2006) J.Physiol. 573, 357.).
- TransfectedHEK-293celllysate(Vicente,R. etal. (2006) J.Biol.Chem. 281, 37675.).
- Ratbrainsections.
- 1.Chandy,K.G.etal.(2001)Toxicon39,1269.
- 2.Koo,G.C.etal.(1997)J.Immunol.158,5120.
- 3.Grissmer,S.etal.(1994)Mol.Pharmacol.45,1227.
- 4.Garcia-Calvo,M.etal.(1993)J.Biol.Chem.268,18866.
- 5.Garcia,M.L.etal.(1994)Biochemistry33,6834.
- 6.Koschak,A.etal.(1998)J.Biol.Chem.273,2639.
- 7.Kalman,K.etal.(1998)J.Biol.Chem.273,32697.
KV1.3belongstothe Shaker familyof voltage-dependentK+ channels. Thechannel,encodedby KCNA3,iswidelyexpressedinthebrain,lungandosteoclastsandinseveralcell populationsofhematopoieticorigin.TheprominenceofKV1.3channelsinthesecells(particularlyinTlymphocytes)directedmuchresearch attention.ItwasfoundthatKV1.3isthemainchannelresponsIBLeformaintainingtherestingpotentialinquiescentcellsandregulatingthe Ca2+ signalingthatisindispensablefornormalTlymphocyteactivation.1,2 BasedonthecentralroleofKV1.3inregulatingtheinitiationofanimmuneresponse, thechannelhasbeenrecognizedasapotentialtargetforimmunosuppressantdrugs.1
KV1.3channelsarepotentlyinhibitedbyseveralvenomouspeptidetoxinsamongthem CharyBDotoxin (2.6nM), Noxiustoxin (1nM), Kaliotoxin (0.65nM), Margatoxin (0.05nM), Agitoxin-1 (1.7nM), Agitoxin-2 (0.004nM), Hongotoxin-1 (0.09nM) and Stichodactylatoxin (0.01nM).3-7
AlomoneLabsispleasedtoofferahighlyspecificantibodydirectedagainstanepitopeofhumanKV1.3.Anti-KV1.3(KCNA3)Antibody(#APC-002)canbeusedinwesternblot,immunoprecipitation,immunocytochemistry,andimmunohistochemistryapplications.IthasbeendesignedtorecognizeKV1.3potassiumchannelfromhuman,rat,andmousesamples.
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多谢!
不知道能不能直接买到大鼠抗豚鼠血管IgG的ELISA试剂盒。
如果买不到这种试剂盒,用空白板的话(就是反应孔中没有抗原或者抗体包被),把自制的豚鼠血管抗原加到反应孔中进行孵育,能不能使抗原附着在孔壁上?这样的话就可以按照elisa的操作步骤进行大鼠血清检测了。
目前的设想是自制抗原,粉碎豚鼠的血管,制成悬液,通过反复多次的冻融,离心后收集上清液作为抗原(即豚鼠血管抗原)。
然后将抗原加入到elisa的反应孔中(这种是特制的空白板,就是反应孔中没有抗原或抗体包被)进行孵育,使豚鼠血管抗原附着在孔壁上,就是让反应孔充当固相载体,形成固相抗原。倒掉多余的抗原。
再加入待检测的大鼠血清,这样血清中的特异性抗豚鼠血管IgG就可以跟固相的抗原结合,形成固相抗原抗体复合物。
加入酶标的兔抗鼠或者羊抗鼠IgG,形成酶标的抗原抗体复合物。
然后就是一些显色步骤。
现在关键的问题就是抗原加入空白板,能不能形成固相抗原,如果不能跟孔壁附着的话,在后面洗涤的过程中就被洗掉了,那就没办法完成检测了。
请各位大侠给指条明路啊!!!小弟在此多谢了!!!