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NewEast/Ras Protein [10110-1][100 µg]/10110-1
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4000-520-616
| CatalogNumber: 10110 | |||||||||||
| ProductName: RasProtein | |||||||||||
| Synonyms: GTPaseRas | |||||||||||
| Source: Human,recombinantfulllength,His6-tag | |||||||||||
| ExpressionHost: E.coli | |||||||||||
| MolecularWeight: 21kDa | |||||||||||
| Purity: >95%bySDS-PAGE | |||||||||||
| Introduction: SmallGTPasesareasuper-familyofcellularsignalingregulators.RasbelongstotheRassub-familyofGTPasesthatregulatecellproliferation,cellmotility,andgenetranscription.GTPbindingincreasestheactivityofRas,andthehydrolysisofGTPtoGDPrendersitinactive.GTPhydrolysisisaidedbyGTPaseactivatingproteins(GAPs),whileexchangeofGDPforGTPisfacilitatedbyguaninenucleotideexchangefactors(GEFs). | |||||||||||
| AminoAcidSequence (1-189) | |||||||||||
MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAM RDQYMRTGEGFLCVFAINNTKSFEDIHQYREQIKRVKDSDDVPMVLVGNKCDLAARTVESRQAQDLA RSYGIPYIETSAKTRQGVEDAFYTLVREIRQHKLRKLNPPDESGPGCMSCKCVLS| Properties | PhysicalAppearance(form): Dissolvedin20mMTris-HCl,pH8.0,150mMNaCl. | PhysicalAppearance(form): Whiteorclear | Concentration: 1mg/mL | Storage: -80°C | PreparationInstructions: Centrifugethevialbeforeopenthecapandreconstituteinwater.Addingof10mMβ-mercaptoethanolor1mMDTTintothesolutiontoprotecttheproteinisrecommendedandusingofnon-ionicdetergentssuchasn-Dodecylβ-D-maltoside(DoDM)orpolyethylenedetergents(e.g.C12E10)alsohelptostABIlizetheprotein.Avoidrepeatedfreezingandthawingafterreconstitution.ThepurityofHis-taggedRaswasdeterminedbySDS-PAGEandCoomassieBrilliantBlueStaining. | ![]() References: 1.Chen,H.-J.etal.,Neuron20:895-904,1998.2.Hamdan,F.F.etal.,NewEng.J.Med.360:599-605,2009.3.Oh,J.S.etal.,Neuron33:151only,2002.4.Rumbaugh,G.etal.,Proc.Nat.Acad.Sci.103:4344-4351,2006.5.Tomoda,T.etal.,GenesDev.18:541-558,2004. | Datasheet: | ![]()
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