Recombinant Interleukin 10 (IL10)
CSIF; IL10A; TGIF; Cytokine Synthesis Inhibitory Factor
- Product No.RPA056Ca01
- Organism SpeciesCanis familiaris; Canine (Dog) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Caprine
- Ovine
- Equine
- Bovine
- Porcine
- Gallus
- Canine
- Others
- Multi-species
- Pan-species
- SourceProkaryotic expression
- HostE.coli
- Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
- Subcellular Locationn/a
- Predicted Molecular Mass49.0kDa
- Accurate Molecular Mass49kDa(Analysis of differences refer to the manual)
- Residues & TagsArg21~Ile181 with N-terminal His and GST Tag
- Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
- TraitsFreeze-dried powder
- Purity> 95%
- Isoelectric Pointn/a
- ApplicationsPositive Control; Immunogen; SDS-PAGE; WB.If bio-activity of the protein is needed, please check active protein.
- DownloadInstruction Manual
- UOM10µg50µg200µg1mg5mg
- FOBUS$ 192 For more details, please contact local distributors!US$ 480 For more details, please contact local distributors!US$ 960 For more details, please contact local distributors!US$ 2880 For more details, please contact local distributors!US$ 7200 For more details, please contact local distributors!
SEQUENCE of the Recombinant Interleukin 10 (IL10)
USAGE of the Recombinant Interleukin 10 (IL10)
Reconstitute in PBS or others.
STORAGE of the Recombinant Interleukin 10 (IL10)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Recombinant Interleukin 10 (IL10)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
Polyacrylamide Gel Electrophoresis (PAGE) Experiment Service
INCREMENT SERVICES
Endotoxin Removal KitBCA Protein Quantification KitProtein Labeling Customized ServiceMolecular Mass Marker for ProteinRecombinant Protein Customized ServiceMonoclonal Antibody Customized ServicePolyclonal Antibody Customized ServiceProtein Activity Test Experiment ServiceImmunoprecipitation (IP) Experiment ServiceBufferEndotoxin Removal Kit IIReal Time PCR Experimental ServiceSpike RBD Protein (S-RBD)Protein GProtein A
Related products
| Catalog No. | Organism species: Canis familiaris; Canine (Dog) | Applications (RESEARCH USE ONLY!) |
| RPA056Ca01 | Recombinant Interleukin 10 (IL10) | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA056Ca61 | Active Interleukin 10 (IL10) | Cell culture; Activity Assays. |
| EPA056Ca61 | Eukaryotic Interleukin 10 (IL10) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA056Ca01 | Polyclonal Antibody to Interleukin 10 (IL10) | WB; IHC; ICC; IP. |
| PAA056Ca06 | Polyclonal Antibody to Interleukin 10 (IL10) | WB; IHC; ICC; IP. |
| MAA056Ca21 | Monoclonal Antibody to Interleukin 10 (IL10) | WB; IHC; ICC; IP. |
| MEA056Ca | Mini Samples ELISA Kit for Interleukin 10 (IL10) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SEA056Ca | ELISA Kit for Interleukin 10 (IL10) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| KSA056Ca01 | ELISA Kit DIY Materials for Interleukin 10 (IL10) | Main materials for "Do It(ELISA Kit) Yourself" |
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最好能说得具体点,小弟对此几乎一窍不通
谢谢
1. 粗纯:将制备抗体的血清或是腹水,细胞上清,直接用盐析法进行处理,这样可以将这些物质里面的其他杂质去掉,获得蛋白的成分,但是由于是粗纯,里面会混有大量的其他蛋白,这样获得的抗体,纯度较低,用于实验中背景比较高。
2.通用型纯化:用抗体结合蛋白Protein A,Protein G或者Protein L。因为不同来源的抗体和这些抗体结合蛋白的结合能力不同,所以需要根据抗体来源选择使用哪种抗体将诶和蛋白最好。对于有一些单链抗体,则多半使用protein L来进行纯化。经过抗体结合蛋白的亲和纯化后,溶液中基本只保留了抗体的成分,其他蛋白都去掉了,抗体纯度可以比较高。相对来说,这种方法是大规模抗体制备中,用得最多的纯化方法,很多抗体公司都采用这种方法来对抗体进行纯化。
3.特异型纯化:但是有些抗体,需要纯度特别高,特异性特别好,就不能简单采用上述两种方法进行纯化了。必须要通过将抗原固定制备成特异的亲和纯化柱,再纯化抗体。这个时候得到的就全是针对一种抗原的抗体了,特异性最好。当然,由于牵涉到抗原固定等操作,成本相应是最高的。
1. 粗纯:将制备抗体的血清或是腹水,细胞上清,直接用盐析法进行处理,这样可以将这些物质里面的其他杂质去掉,获得蛋白的成分,但是由于是粗纯,里面会混有大量的其他蛋白,这样获得的抗体,纯度较低,用于实验中背景比较高。
2.通用型纯化:用抗体结合蛋白Protein A,Protein G或者Protein L。因为不同来源的抗体和这些抗体结合蛋白的结合能力不同,所以需要根据抗体来源选择使用哪种抗体将诶和蛋白最好。对于有一些单链抗体,则多半使用protein L来进行纯化。经过抗体结合蛋白的亲和纯化后,溶液中基本只保留了抗体的成分,其他蛋白都去掉了,抗体纯度可以比较高。相对来说,这种方法是大规模抗体制备中,用得最多的纯化方法,很多抗体公司都采用这种方法来对抗体进行纯化。
3.特异型纯化:但是有些抗体,需要纯度特别高,特异性特别好,就不能简单采用上述两种方法进行纯化了。必须要通过将抗原固定制备成特异的亲和纯化柱,再纯化抗体。这个时候得到的就全是针对一种抗原的抗体了,特异性最好。当然,由于牵涉到抗原固定等操作,成本相应是最高的。


