
Fast-n-Pure Plasmid Kit (Ver. 2.0)
- Simplified and fast method (2 steps, 10 min)
- Spin column based
- Up to 20 ug DNA/column
- Sequencing and transfection grade
Products
Cat.No. | Product | Size |
---|---|---|
FPL50 | PureHelix™ Fast-n-Pure Plasmid Kit (Ver. 2.0) | 50 preps |
FPL200 | PureHelix™ Fast-n-Pure Plasmid Kit (Ver. 2.0) | 200 preps |
- Description
- Data
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PureHelix™ Fast-n-Pure Plasmid Kit is a simplified and fast kit than the conventional method for isolation of pure plasmids up to 20 ug for routine molecular biological applications, such as PCR, cloning, sequencing, in vitro transcription, transfection experiments, etc. PureHelix™ Fast-n-Pure Plasmid Kit is based on alkaline-lysis method and the binding properties of DNA on silica-gel membrane to provide the high-quality and high-yield plasmid DNAs. Especially, PureHelix™ Fast-n-Pure Plasmid Kit is also excellent to isolate low-copy plasmids and large size of circular DNAs, such as fosmids and recombinant BAC plasmids.
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非常急!
蛋白质在水溶液中的溶解度是由蛋白质周围亲水基团与水形成水化膜的程度,以及蛋白质分子带有电荷的情况决定的。当用中性盐加入蛋白质溶液,中性盐对水分子的亲和力大于蛋白质,于是蛋白质分子周围的水化膜层减弱乃至消失。同时,中性盐加入蛋白质溶液后,由于离子强度发生改变,蛋白质表面电荷大量被中和,更加导致蛋白溶解度降低,使蛋白质分子之间聚集而沉淀。一般33%硫酸铵为沉淀Ig-G的最适饱和度
有机溶剂沉淀,极性有机溶剂降低介点常数使蛋白质凝集沉淀。eg.甲醇、乙醇、丙酮。
磺基水杨酸也可以沉淀蛋白质,但要加酸调节ph,使之低于血清蛋白的pi,使蛋白质带正电荷,与水杨酸酸根结合生成不溶盐而沉淀。
血清蛋白质均带负电荷,但各种蛋白质带负电荷的程度有所差异,以白蛋白为最多。而血清白蛋白等电点为4.7-4.9。所以把ph调到4.7以下就可以了。

