
BioVortexer
The BioVortexer™ is a battery-powered motor drive designed to rotate stirring rods or plastic pestles in 1.5 ml microtubes. Rotating at thousands of revolutions per minute, it makes short work of dispersing centrifugal pellets or grinding small tissue samples.
Cat. No. 1083MC, BioVortexer Stirring Kit. Includes motor, batteries and 100 - 1/8"X3" stirring rods.
Cat. No. 1083, BioVortexer, motor only. Batteries included.
Cat. No. 183, Additional BioVortexer polypropylene stirring rods, 1/8"X3" (500 count).
Cat. No. 1017MC, BioVortexer Grinding Kit. Includes motor, batteries, 100 SpiralPestles™ with matching 1.5 ml microvials and sample of SpiralPestle™ Grinding Beads.
Cat. No. 1017, Additional SpiralPestles™ with matching 1.5 ml microvials (100 count) and sample of SpiralPestle™ Grinding Beads.
Our Price : $0.00
The BioVortexer has two distinct applications...
- A practical alternative to a conventional vortex mixer for mixing and dispersing samples. Combined with Disposable Polypropylene Stirring Rods (1/8 X 3")*, it rapidly re-suspends precipitates such as pellets of protein or DNA. The working range is 10-500 mg of powdered or homogenized solids in a media volume of 0.5 to 10 ml. "Take the BioVortexer to the sample instead of the sample to the vortex mixer".
- A low cost device to rapidly homogenizing small samples of soft tissue for extraction of proteins and nucleic acids. The BioVortexer rotates Disposable Polypropylene SpiralPestles™ at ~9000 rpm in 0.6 or 1.5 ml conical microtubes containing sample, extraction media and a small quantity of glass grinding beads. Complete cell lysis of soft tissue samples is achieved in seconds. See SpiralPestle for more information on this new method.
- Rotates 3" Stirring Rods or Pestles in small tubes or microvials for rapid, efficient mixing or grinding of pellets or soft tissue
- Roration speed about.9000 rpm
- Lightweight
- Cordless: Uses two standard AA batteries
- Low cost
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结合物垫在层析时荧光微球在T线前面凝集的现象怎么解决呢,结合物垫增加了吐温和糖的含量还是有这种现象的
同原剂作用由氯金酸(HAuCL4)制备金颗粒直径0.8-500nm胶体金制备胶体金保存间较4℃保存6月或室温保存1-2月
胶体金做标记探针同用途选用胶体金直径范围同用于免疫快速检测胶体金颗粒直径范围般3-40nm间
胶体金粒表面层AuC12—粒表面带负电荷金颗粒表面包层物(蛋白)稳定保护金颗粒维持胶体稳定防止外电解质影响使粒相互凝聚
胶体金粒蛋白吸附作用取决于溶液pH值蛋白氨基酸净电荷取决于溶液pH值pH=pI蛋白溶液呈性pH=pI蛋白溶解度水化程度更容易吸附疏水金粒表面实际胶体金探针制备般胶体金调整pH=pI+0.5更利于结合更稳定
胶体金探针所用蛋白通三种机制于吸附于金颗粒表面:、金粒所带负电荷与蛋白部碱性氨基酸(赖氨酸精氨酸pH均于10)所带阳性电荷初相互吸引;二、蛋白通某些疏水性氨基酸残基(包括色氨酸)与金粒表面间疏水吸附作用;三、蛋白半胱氨酸或甲硫氨酸硫基与金粒间电共用共价键结合
用于制备胶体金探针蛋白需要进行前处理才能与金颗粒更结合未经处理蛋白质般说均含较高浓度盐高浓度盐往往干扰蛋白与胶体金吸附结合或导致胶体金粒凝聚所首先要除蛋白质溶液盐冻干蛋白或高浓度蛋白溶液蛋白凝聚聚同与胶体金粒结合影响探针灵敏度散蛋白单体蛋白前处理重要项处理标记使其蛋白具适量蛋白量(30kD)形蛋白复合体往往稳定量蛋白与其蛋白(BSA牛血清蛋白等)结合能制备稳定性更佳探针量影响探针灵敏度已知蛋白结构与性前提除性影响结构部提高标记灵敏度
二、免疫胶体金产品原理及应用
胶体金探针用单克隆抗体标记应用免疫层析制快速诊断产品具较高特异性且相稳定性高检测般5-10min读结相比其(ELISA需1-2hPCR需要间更)缩短检测间检测(组织液、血清、尿液、粪便等)需做非简单处理或做前处理即进行检测结颜色变化读取需要特别仪器设备
免疫胶体金快速检测试纸利用层析原理、双抗体夹或免疫竞争或间接应用检测物质特异性能与其发特异反应抗原或抗体并金颗粒显色剂达快速检测目
使用快速检测产品卡加孔加入待测利用层析原理断向另端层析随着层析进行本待测固定于测试线(T线)并颜色变化显示并照线(即控制线C线)确保检测效性
建议 这没有意义
也可以自己查阅资料
Rainbow Calibration ParticlesSPHEROBD,但惊奇地发现BD这个目录里面居然也家sphero的rainbow微球,两者啥关系?Life Flow Cytometer Alignment & Linearity BeadsBangs LabThermo ScientificPolysicence Flow Check® Calibration Particles & Kits


