ImmunoTek™ TMB Stable Liquid Substrate 1.56mM for HRPO (3,3',5,5'-Tetramethylbenzidine)||TMB produces a soluble end product which is blue in color that is quantitated at 650nm. Upon addition of an equal volume of 0.5M/Liter HCl, the resulting yellow chromogen is quantitated at 450nm. The yellow product is stable at room temperature for at least 1 hour displaying less than a 1% loss in absorbance during this time interval. 1.56mM TMB is recommended for manual assays.||Benzidine and its derivatives are well documented electron donors for the horseradish peroxidase (HRP) /H2O2 oxidation/reduction system. These amines are known to be potent carcinogens.||The synthesis of TMB by Holland, et al., provided a reagent more sensitive than benzidine and much less hazardous due to ortho methylation.||Using H2O2 as substrate, the reaction of TMB with HRP proceeds through 2 phases. First, a charge transfer complex, the result of a 1-electron oxidation is formed. This is a blue product displaying an absorption maximum at 650nm. Further oxidation proceeds through a green color shift to the fully oxidized yellow diimine with an absorption maximum at 450nm.||The diimine is quantitatively formed only if the molar concentration of H2O2 is twice that of TMB. The molar extinction coefficient of the charge transfer complex is reported to be 3.9x10e4 mol-1 cm-1 and that of the diimine to be 5.9x10e4 mol-1 cm-1.The blue reaction product can be converted to the diimine non-enzymatically by the addition of acid providing an increase in sensitivity of at least 1.5.||1.56mM/Liter TMB is designed to measure low levels of HRP preventing the formation of the diimine allowing the user to determine enzyme activity or analyte concentration kinetically at 650nm or as an end point at 450nm after the addition of acid.Features:|• Single component system|• Room temperature stable|• Exhibits low background levels|• Safe to use. No weighing of hazardous powders|• Save preparation time. Use right out of the bottle|• Ultra-low sensitivity|• Minimal lot-to-lot variation||Appearance: Colorless to pale yellow/orange||Stability (18-26°C): Passes||Stability (4°C): Passes||pH: 4.9 ± 0.03||Storage and Stability: Stable for 18 months at RT. To extend the stability of product, refrigerate at 4°C.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

USBIo全称United States BIOLOGical，位于麻省斯万普斯科特(Swampscott, MA)。全美**的抗原抗体和生化试剂供应商，目前向全球六十余个国家的科研工作者提供约50,000种抗体、抗原和生化试剂，以质量**闻名。主要产品种类包括：抗体、生化试剂、基因克隆相关产品、培养基、生长因子等细胞因子、分子生物学试剂。美国US Biological公司是生物化学和生物学试剂的专业生产商，产品服务于免疫学，分子生物学，分子遗传学，体外诊断，组织培养等科研领域。产品精确度高，质量稳定，产品数量巨大，提供70,000多种抗体、抗原和生化试剂等。

Usbio的生物化学品，抗体，重组蛋白，细胞培养基和分子生物学试剂盒几乎用于所有科学应用和环境，包括基因组研究，生物技术，药物开发和疾病诊断。USBio产品从经济实惠的研究数量到更大的批量可用于工艺开发和生产。我们的客户包括位于美国和国外的大多数*的制药和生物技术公司。

USbiological公司致力于以价值，诚信和真正的个人购买体验降低研究成本。USbiological的生物化学，抗体，重组蛋白，细胞培养基和分子生物学试剂盒几乎用于所有科学应用和环境，包括基因组研究，生物技术，药物开发和疾病诊断。产品可以从负担得起的研究数量到大批量用于工艺开发和生产。

USBio（UnitedStatesBiological，或简称USBiological）位于麻省斯万普斯科特(Swampscott,MA)。作为全美最大的抗原抗体和生化试剂供应商，USBiological目前向全球六十余个国家的科研工作者提供约150,000种抗体、抗原和生化试剂，以质量卓越闻名。主要产品种类包括：抗体、生化试剂、基因克隆相关产品、培养基、生长因子等细胞因子、分子生物学试剂。美国USBiological的产品服务于免疫学，分子生物学，分子遗传学，体外诊断，组织培养等科研领域。USBiological一直恪守“为您的研究减少成本”原则，提供高纯度、高品质的产品，并在大多数生化试剂、生物与培养基产品线上保持最高的性价比。USBiological的产品被广泛用于各种科学研究领域，包括基因组研究、生物技术、药物研发和疾病诊断等。USBiological的产品从小型科研级到生物制品大规模级规格均有提供。众多全球顶ji的生物制药和生物技术公司都在使用USBiological的高品质产品。

usbio的生化试剂，抗体，重组蛋白，细胞培养基和分子生物学试剂盒几乎用于所有科学应用和环境，包括基因组研究，生物技术，药物开发和疾病诊断。

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