96-well Cell Adhesion Assay
(Vitronectin coated, Colorimetric Assay)
Catalog Number: ECM-1014, 72 assays
Storage: Upon arrival store the components at 4C.
Introduction: Due to its diverse nature and composition, the ECM can serve many functions, such as providing support and anchorage for cells, segregating tissues from one another, and regulating intercellular communication. The ECM regulates a cell"s dynamic behavior. In addition, it sequesters a wide range of cellular growth factors, and acts as a local depot for them. Changes in physiological conditions can trigger protease activities that cause local release of such depots. This allows the rapid and local growth factor-mediated activation of cellular functions, without de novo synthesis.
Formation of the extracellular matrix is essential for processes like growth, wound healing and fibrosis. An understanding of ECM structure and composition also helps in comprehending the complex dynamics of tumor invasion and metastasis in cancer biology as metastasis often involves the destruction of extracellular matrix[3] by enzymes such as serine and Threonine proteases and Matrix metalloproteinase.
BioPioneer Cell Adhesion Microplate Assays are designed for the determination of the relative attachment of adherent cell lines to extracellular matrix proteins such as Human Fibronectin, Human Vitronectin, Human Collagen IV and Laminin.
Principle of the assay
BioPioneer 96-well Cell Adhesion Assays are designed for the determination of cell attachment to ECM proteins. After incubation followed by a brief wash step, attached cells are quantified with the dye. BSA-coated wells serve as a negative control, and poly-L-lysine-coated wells serve as a positive control for general attachment.
Kit Components
1. Vitronectin Adhesion Plate (Part No. ECM-10114): One 96-well plate containing 72 Bovine Laminin coated wells , 8 BSA-coated wells and 8 poly-lysine coated wells(see layout below)
2. Cell Stain Solution (Part No. 10001): One Bottle10.0ml
3. Extraction Solution (Part No. 10002): One Bottle 10.0ml
Materials Not Supplied
1. Cell culture medium
2. Serum free medium, such as DMEM containing 0.5% BSA, 2 mM CaCl2 and 2 mM MgCl2
4. 1X PBS
5. 96-well microtiter plate
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（1）水浴式PCR仪：变温快、时间准、温度均一，但体积大，自动化程度不高。
（2）变温金属块式PCR仪：通过半导体加热和冷却，并由微机控制恒温和冷热处理过程。装置比较牢固耐用，温度变换平稳，有利于保持Taq DNA聚合酶的活性。
（3）变温气流式PCR仪：以空气作为热传播媒介，由大功率风扇及制冷设备提供外部冷空气而制冷。成本较低；安全程度高。微机配上软件，可灵活编程。
求Hema9600基因扩增仪用户手册，实验室有这个仪器，官网（http://www.zhhema.com/zh-CN/displayproduct.html?proID=100438154）找不到说明书。
308C是16孔道的，单次反应可检测1-14项不同指标。
要做滚环扩增，实验室没有荧光光谱仪，可以用荧光定量PCR仪代替吗？
