BP-FectinTM mammalian cell transfection reagent is a proprietary formulation of cationic polymers optimized for high transfection efficiency, low cytotoxicity, and ease of use. BP-FectinTM transfection reagent is a superior alternative to a number of other transfection techniques including calcium phosphate coprecipitation, electroporation, microinjection, biolistic particle delivery, and complex formation with DEAE-dextran. BP-FectinTM transfection reagent offers excellent performance in both stable and transient transfection of both mammalian and insect cells. 2x1.5ml of BP-FectinTM transfection reagent provides enough reagent to perform up to 400 x 1ug transfections. BP-FectinTM transfection reagent is supplied as a ready-to-use sterile solution and is Ideal for high-throughput transfection in a multi-well plate format.
Advantages High transfection efficiency for both adherent and suspension cells.
High transfection efficiency for both stable and transient transfections.
High transfection efficiency in both serum-containing and serum-free media.
Simple and easy to use with highly reproducibility.
No need to remove media after transfection.
Minimal cytotoxicity.
Storage Store BP-FectinTM mammalian cell transfection reagent at 4C.
Cell Types The following cell lines are successfully transfected by BPFectin: COS-&, HepG2, MCF7, PC12, CHO, VERO, MDCK<MDBK,and293 cells
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求Hema9600基因扩增仪用户手册，实验室有这个仪器，官网（http://www.zhhema.com/zh-CN/displayproduct.html?proID=100438154）找不到说明书。
308C是16孔道的，单次反应可检测1-14项不同指标。
要做滚环扩增，实验室没有荧光光谱仪，可以用荧光定量PCR仪代替吗？
（1）水浴式PCR仪：变温快、时间准、温度均一，但体积大，自动化程度不高。
（2）变温金属块式PCR仪：通过半导体加热和冷却，并由微机控制恒温和冷热处理过程。装置比较牢固耐用，温度变换平稳，有利于保持Taq DNA聚合酶的活性。
（3）变温气流式PCR仪：以空气作为热传播媒介，由大功率风扇及制冷设备提供外部冷空气而制冷。成本较低；安全程度高。微机配上软件，可灵活编程。
