
Product Name | SensoLyte ® 520 Thrombin Activity Assay Kit *Fluorimetric* |
Size | 1 kit |
Catalog # | AS-72129 |
US$ | $526 |
Thrombin, a serine protease, plays a central role in hemostasis by converting soluble plasma fibrinogen into an insoluble fibrin clot and by promoting platelet aggregation.Thrombin also influences a number of normal and pathological processes, including inflammation, tissue repair, embryogenesis, angiogenesis, and tumor invasion.The SensoLyte® 520 Thrombin Assay Kit is optimized for detecting thrombin activity.This kit contains a novel internally quenched 5-FAM/QXL® 520 FRET substrate for thrombin.Thrombin cleaves the FRET substrate into two separate fragments resulting in the release of 5-FAM fluorescence which can be monitored at excitation/emission= 490 nm/520 nm.The long wavelength fluorescence of 5-FAM is less interfered by the autofluorescence of components in biological samples and test compounds.The assay can be performed in a convenient 96-well or 384-well microplate format for screening of enzyme inhibitors. Kit size: 100 assays. | |
Detailed Information | ![]() ![]() |
Product Citations | Shin SJ, et al. (2020) Role of PAR1-Egr1 in the initiation of thoracic aortic aneurysm in Fbln4-deficient mice. Arteriosclerosis, Thrombosis, and Vascular Biology. 40(8):1905-17. doi: 10.1161/ATVBAHA.120.314560.Li, Y.T., et al (2016). Platelet-cytokine Complex Suppresses Tumour Growth by Exploiting Intratumoural Thrombindependent Platelet Aggregation Sci. Rep. DOI: 10.1038/srep25077Aleman-Garcia, MA. (2014). Ion-Responsive HeminG-Quadruplexes for Switchable DNAzyme and Enzyme Functions. Chem Eur J 20, 5619. doi: 10.1002/chem.201304702.Mogami, H. et al. (2014). Effect of thrombin on human amnion mesenchymal cells, mouse fetal membranes, and preterm birth.J Biol Chem 289, 13295. doi: 10.1074/jbc.M114.550541.Zheng, C. et al. (2014). System-level multi-target drug discovery from natural products with applications to cardiovascular diseases. Mol Diversity 1. doi:10.1007/s11030-014-9521-yMartini, C. et al. (2012). Antimicrobial and Anticoagulant Activities of N-Chlorotaurine, N,N-Dichloro-2,2-Dimethyltaurine, and N-Monochloro-2,2-Dimethyltaurine in Human Blood. Antimicrob. Agents Chemother 56; 4 1979.doi: 10.1128/AAC.05685-11Gugerell, A. et al. (2012). High thrombin concentrations in fibrin sealants induce apoptosis in human keratinocytes. J Biomed Mater Res A. 100A: 1239. doi: 10.1002/jbm.a.34007Luan, NM. et al. (2011). Layer-by-layer co-immobilization of soluble complement receptor 1 and heparin on islets. Biomaterials. 32: 6487. doi:10.1016/j.biomaterials.2011.05.048Chen, H. et al. (2011). Immobilization of anticoagulant-loaded liposomes on cell surfaces by DNA hybridization. Biomaterials. 32: 7971.doi:10.1016/j.biomaterials.2011.07.002Shiang, YC. et al. (2010). Aptamer-conjugated nanoparticles efficiently control the activity of thrombin. Adv Funct Mater 20, 3175. doi: 10.1002/adfm.201000642. |
ebiomall.com






>
>
>
>
>
>
>
>
>
>
>
>
求Hema9600基因扩增仪用户手册,实验室有这个仪器,官网(http://www.zhhema.com/zh-CN/displayproduct.html?proID=100438154)找不到说明书。
要做滚环扩增,实验室没有荧光光谱仪,可以用荧光定量PCR仪代替吗?
308C是16孔道的,单次反应可检测1-14项不同指标。
(1)水浴式PCR仪:变温快、时间准、温度均一,但体积大,自动化程度不高。
(2)变温金属块式PCR仪:通过半导体加热和冷却,并由微机控制恒温和冷热处理过程。装置比较牢固耐用,温度变换平稳,有利于保持Taq DNA聚合酶的活性。
(3)变温气流式PCR仪:以空气作为热传播媒介,由大功率风扇及制冷设备提供外部冷空气而制冷。成本较低;安全程度高。微机配上软件,可灵活编程。

