- Product Name14-3-3 beta/zeta Colorimetric Cell-Based ELISA Kit
- Catalog NoCB5001
- Detection MethodColorimetric 450 nm
- Dynamic Range> 5000 Cells
- Storage/Stability4°C/6 Months
- ReactivityHuman, Mouse, Rat
- Assay TypeCell-Based
- Database LinksGene ID: 7534/7529, UniProt ID: P63104/P31946, OMIM #: 601288/601289, Unigene #: Hs.492407/Hs.594673/Hs.643544
- Format96-Well Microplate
- Manual (PDF)<a href="image/catalog/assbiotech/CB5001-2018.pdf">Download</a>
- NCBI Gene SymbolYWHAB/YWHAZ
- Sub TypeNone
- Synonyms14-3-3 protein zeta/delta, 1433Z, 143Z, FAS, Factor activating exoenzyme S, KCIP-1, Mitochondrial import stimulation factor S1 subunit, Protein kinase C inhibitor protein-1, YWHAZ
- TargetName14-3-3 beta/zeta
AssayBiotechnology公司成立于2006年,位于美国洛杉矶。AssayBiotechnology公司有超过六千种抗体,涵盖了各种研究和应用领域。另外,AssayBiotechnology公司还拥有一千多种磷酸化抗体,同时公司正努力生产所有人类genowide抗体,针对到2015年为止出版过的调控磷酸化位点的抗体。作为可靠的高质量抗体生产商,AssayBiotechnology公司拥有多年研发经验的高级科学家确保了公司抗体的高品质,高特异性,100%的保证抗体质量。
AssayBiotechnology公司利用主要由混合抗原均一化技术和人类cDNA/EST噬菌体表面呈现文库技术两项具有自主知识产权的专有技术组成抗人类蛋白单克隆抗体大规模开发生产平台,完美地解决了传统的单克隆抗体生产耗时过长及成本过高的瓶颈。AssayBiotechnology公司可在较短的时间内将剩余的抗人类基因编码蛋白质单克隆抗体开发完,从而建立世界上第一个与人类基因库相配套的抗人类蛋白单克隆抗体库和抗原库(该抗体库和抗原库与国际上通用的GenBank相链接),并建成全球种类最多和规模最大的抗人类蛋白单克隆抗体生产体系和销售中心。
与人类基因相联系的抗体蛋白与抗原蛋白信息是有限的,并且每一个抗体与抗原蛋白相关信息都是唯一的和不可复制的,和人类基因组计划一样,谁先开发出来谁就拥有垄断优势。AssayBiotechnology公司开发出抗体库和抗原库之后,将对每一个新发现的和有价值的抗体与抗原信息申请专利保护,以后所有应用到这些抗体与抗原信息的需求者(例如药物靶点筛选)都必须向AssayBiotechnology公司购买使用许可,因而这是最新生命科学领域的战略高地。
自2006年1月成立以来,AssayBiotechnology一直是行业*的抗体生产和工程及分析技术,荧光染料,淬灭剂,重组蛋白和合成肽的全球贡献者。我们位于加利福尼亚州旧金山湾区的中心地带,致力于满足全球对医疗和学术研究中使用的高质量检测试剂盒和免疫试剂的需求,这些试剂主要构成医疗保健的基础。详细产品列表:AssayBiotech全国代理 货号产品规格OK-01004-1BB-R(Human)OmniKine™ELISAKit12x8-WellMicrostripsOK-0101BD-1(Human)OmniKine™ELISAKit12x8-WellMicrostripsOK-0102BD-2(Human)OmniKine™ELISAKit12x8-WellMicrostripsOK-0103BD-3(Human)OmniKine™ELISAKit12x8-WellMicrostripsOK-0104BD-4(Human)OmniKine™ELISAKit12x8-WellMicrostripsOK-0105Betacellulin(Human)OmniKine™ELISAKit12x8-WellMicrostripsOK-0106beta-NGF(Human)OmniKine™ELISAKit12x8-WellMicrostripsOK-0107BMP-2(Human)OmniKine™ELISAKit12x8-WellMicrostripsOK-0108CNTF(Human)OmniKine™ELISAKit12x8-WellMicrostripsOK-0109CTGF(Human)OmniKine™ELISAKit12x8-WellMicrostripsOK-0110CXCL-16(Human)OmniKine™ELISAKit12x8-WellMicrostripsOK-0111EGF(Human)OmniKine™ELISAKit12x8-WellMicrostripsOK-0112EG-VEGF(Human)OmniKine™ELISAKit12x8-WellMicrostripsOK-0113Eotaxin(Human)OmniKine™ELISAKit12x8-WellMicrostripsOK-0114Eotaxin-3(Human)OmniKine™ELISAKit12x8-WellMicrostripsOK-0115FGF-Basic(Human)OmniKine™ELISAKit12x8-WellMicrostripsOK-0116Follistatin(Human)OmniKine™ELISAKit12x8-WellMicrostripsOK-0117G-CSF(Human)OmniKine™ELISAKit12x8-WellMicrostripsOK-0118GM-CSF(Human)OmniKine™ELISAKit12x8-WellMicrostripsOK-0119GRO/MGSA(Human)OmniKine™ELISAKit12x8-WellMicrostripsA000114-3-3zeta(Phospho-Ser58)Antibody50ulA0002ADD1(Phospho-Ser726)Antibody50ulA0003AMPKalpha1/2(Phospho-Thr183/172)Antibody50ulA0004AmyloidbetaA4(Phospho-Thr743/668)Antibody50ulA0005CaMK2alpha/beta/delta(Phospho-Thr305)Antibody50ulA0006CREB(Phospho-Ser142)Antibody50ulA0007DARPP-32(Phospho-Thr75)Antibody50ulA0008EGFR(Phospho-Thr678)Antibody50ulA0009EGFR(Phospho-Thr693)Antibody50ulA0010EFNB1/2(Phospho-Tyr330)Antibody50ulA0011GABA-RB(Phospho-Ser434)Antibody50ulA0012GSK3alpha/beta(Phospho-Tyr279/216)Antibody50ulA0013HSP90B(Phospho-Ser254)Antibody50ulA0014IkappaB-beta(Phospho-Ser23)Antibody50ulA0015IkappaB-epsilon(Phospho-Ser22)Antibody50ulA0016Keratin18(Phospho-Ser33)Antibody50ulA0017Keratin8(Phospho-Ser73)Antibody50ulA0018MAPKAPK2(Phospho-Thr334)Antibody50ulA0019MEF2A(Phospho-Ser408)Antibody50ulA0020Myc(Phospho-Ser62)Antibody50ul
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生物偶联即将两种分子结合在一起而形成一个杂合的偶联物(hybridconjugate),这些偶联结合物(如标记抗体)在世界各大医院和科研机构被广泛使用。一抗标记试剂系列产品极大程度的简化了结合标记流程,是目前世界上操作最简便的抗体标记试剂盒。
·快速:手工操作时间仅30秒·目前世界上操作最简便的抗体标记试剂盒·无需脱盐、透析等步骤·抗体回收率达100%·可提供数十种标记物·可用于WB,IHC,ELISA,免疫荧光、流式细胞术以及荧光共振能量转移检测等·经济实惠,规格多样·可标记ug-mg级抗体量试剂盒组成:
1)1或3(以规格而定)玻管Lightening-Link?Mix混合物。
2)1管LL-X(X为标记物,如PE或HRP等)修饰试剂。
3)1管LL-X萃灭试剂。
操作步骤:
1)在每10ul抗体样品中加入1ul的LL-Modifier修饰试剂,轻轻混匀。
2)打开Ligtening-Link混合物管盖,吸取步骤1已混好的抗体样品至管内冻干混合物中,用吸液器轻轻吸打1-2次,使粉末完全溶解。
3)盖上玻璃瓶盖,室温(20-25°C)避光孵育3小时,或过夜。*
4)孵育3小时后,按每10ul抗体的量加入1ulLL-淬灭试剂,静置30分钟后即可使用。
*不同的标记物,操作步骤略有不同。(操作步骤供参考,以英文原版说明书为准。)
标记结合物的保存:
对于任何新结合物,推荐保持于4°C条件下。如需长期保存,可添加保护剂。
缓冲液及添加剂兼容性:
A.与无胺基缓冲液兼容,包括MES,MOPS,HEPES(浓度10-50mM,pH范围6.5-8.5),LL-Modifier修饰剂提供结合反应的最佳条件。
B.常见的盐(如氯化钠)、螯合剂(EDTA)、糖对偶联反应无影响。
C.叠氮钠(0.02%-0.1%)对结合反应有轻微影响,叠氮钠不可用于HRP标记,其为后者的抑制剂。
D.甘油(upto50%)对结合效率无影响。
E.若无胺基的缓冲液浓度相对较高,pH值在6.6-8.5范围外,则需要加入更多的LL-midifier修饰剂(/10ul抗体),以此来调整pH值。最佳的pH范围为7.3-7.6左右。
F.避免使用含亲核剂的缓冲液,避免还原剂如DTT和2-巯基乙醇,乙醇胺等。
G.浓度达到或稍低于20mM的Tris对结合效率有轻微的影响。
Westernblottingdatacomparingconjugatedandunconjugatedantibodies
货号中文品名英文品名规格AZK0001AColorlockALS磷检测试剂盒PiColorlockALS625/1560assaysAZK0001BColorlockALS磷检测试剂盒PiColorlockALS2500/6250assaysAZK0002AColorlockGold磷检测试剂盒PiColorlockGold625/1560assaysAZK0002BColorlockGold磷检测试剂盒PiColorlockGold2500/6250assaysAZK0003APi结合树脂PiBind5gAZK0004AATP琼脂糖Low(1-2umol/ml)ATPagaroseLow(1-2umol/ml)2mlAZK0004BATP琼脂糖Low(1-2umol/ml)ATPagaroseLow(1-2umol/ml)5mlAZK0005AATP琼脂糖High(>6umol/ml)GTPagaroseHigh(>6umol/ml)2mlAZK0006AATP琼脂糖High(1-2umol/ml)GTPagaroseHigh(>6umol/ml)5mlAZK0007AATP琼脂糖High(8-12umol/ml)ATPagaroseHigh(8-12umol/ml)2mlAZK0008AATP琼脂糖High(8-12umol/ml)ATPagaroseHigh(8-12umol/ml)5mlAZK0009A控制树脂ControlResin2mlAZK0009B控制树脂ControlResin5mlAZK0010AATPase酶检测试剂盒ATPaseassaysystem192assaysAZK0010BATPase酶检测试剂盒ATPaseassaysystem480assaysAZK0010CATPase酶检测试剂盒ATPaseassaysystem5x480assaysAZK0011AGTPase酶检测试剂盒GTPaseassaysystem192assaysAZK0011BGTPase酶检测试剂盒GTPaseassaysystem480assaysAZK0011CGTPase酶检测试剂盒GTPaseassaysystem5x480assaysAZK0013AAP碱性磷酸酶抗体标记试剂盒Lightning-LinkAPconjugationkit3x10μgAPAZK0013BAP碱性磷酸酶抗体标记试剂盒Lightning-LinkAPconjugationkit1x100μgAPAZK0013CAP碱性磷酸酶抗体标记试剂盒Lightning-LinkAPconjugationkit3x100μgAPAZK0013DAP碱性磷酸酶抗体标记试剂盒Lightning-LinkAPconjugationkit1mgAPAZK0014AGOx葡糖氧化酶标记试剂盒Lightning-LinkGOxconjugationkit3x10μgAZK0014BGOx葡糖氧化酶标记试剂盒Lightning-LinkGOxconjugationkit3x100μgAZK0014CGOx葡糖氧化酶标记试剂盒Lightning-LinkGOxconjugationkit1x1mgAZK0015AR-PE(PE)R-藻红蛋白标记试剂盒Lightning-LinkR-Phycoerythrin(R-PE)3x10μgPEAZK0015BR-PE(PE)R-藻红蛋白标记试剂盒Lightning-LinkR-Phycoerythrin(R-PE)1x100μgPEAZK0015CR-PE(PE)R-藻红蛋白标记试剂盒Lightning-LinkR-Phycoerythrin(R-PE)3x100μgPEAZK0015DR-PE(PE)R-藻红蛋白标记试剂盒Lightning-LinkR-Phycoerythrin(R-PE)1mgPEAZK0016AB-PEB-藻红蛋白标记试剂盒Lightning-LinkB-Phycoerythrin(B-PE)1x100μgBPEAZK0016BB-PEB-藻红蛋白标记试剂盒Lightning-LinkB-Phycoerythrin(B-PE)3x100μgBPEAZK0016CB-PEB-藻红蛋白标记试剂盒Lightning-LinkB-Phycoerythrin(B-PE)1mgBPEAZK0017AAPC别藻蓝蛋白标记试剂盒Lightning-LinkAllophycocyanin(APC)3x10μgAPCAZK0017BAPC别藻蓝蛋白标记试剂盒Lightning-LinkAllophycocyanin(APC)1x100μgAPCAZK0017CAPC别藻蓝蛋白标记试剂盒Lightning-LinkAllophycocyanin(APC)3x100μgAPCAZK0017DAPC别藻蓝蛋白标记试剂盒Lightning-LinkAllophycocyanin(APC)1mgAPCAZK0018APerCP标记试剂盒Lightning-LinkPerCP3x10ugAZK0018BPerCP标记试剂盒Lightning-LinkPerCP1x100ugAZK0018CPerCP标记试剂盒Lightning-LinkPerCP3x100ugAZK0018DPerCP标记试剂盒Lightning-LinkPerCP1x1mgAZK0019A荧光素标记试剂盒Lightning-LinkFluorescein3reactions(eachupto20ug)AZK0019B荧光素标记试剂盒Lightning-LinkFluorescein3reactions(eachupto200ug)AZK0019C荧光素标记试剂盒Lightning-LinkFluorescein1reaction(upto2mg)AZK0020A罗丹明标记试剂盒Lightning-LinkRhodamine3reactions(eachupto20ug)AZK0020B罗丹明标记试剂盒Lightning-LinkRhodamine3reactions(eachupto200ug)AZK0020C罗丹明标记试剂盒Lightning-LinkRhodamine1reaction(upto2mg)AZK0021AAMCA标记试剂盒Lightning-LinkAMCA3reactions(eachupto20ug)AZK0021BAMCA标记试剂盒Lightning-LinkAMCA3reactions(eachupto200ug)AZK0021CAMCA标记试剂盒Lightning-LinkAMCA1reaction(upto2mg)AZK0022ATexasRed标记试剂盒Lightning-LinkTexasRed3reactions(eachupto20ug)AZK0022BTexasRed标记试剂盒Lightning-LinkTexasRed3reactions(eachupto200ug)AZK0022CTexasRed标记试剂盒Lightning-LinkTexasRed1reaction(upto1mgscale)AZK0023ACy3染料标记试剂盒Lightning-LinkCy33reactions(eachupto20ug)AZK0023BCy3染料标记试剂盒Lightning-LinkCy33reactions(eachupto200ug)AZK0023CCy3染料标记试剂盒Lightning-LinkCy31reaction(upto2mg)AZK0024ACy5染料标记试剂盒Lightning-LinkCy53reactions(eachupto20ug)AZK0024BCy5染料标记试剂盒Lightning-LinkCy53reactions(eachupto200ug)AZK0024CCy5染料标记试剂盒Lightning-LinkCy51reaction(upto2mg)AZK0025ACy5.5染料标记试剂盒Lightning-LinkCy5.53reactions(eachupto200ug)AZK0025BCy5.5染料标记试剂盒Lightning-LinkCy5.51reaction(upto2mg)AZK0026ACy7染料标记试剂盒Lightning-LinkCy73reactions(eachupto20ug)AZK0026BCy7染料标记试剂盒Lightning-LinkCy73reactions(eachupto200ug)AZK0026CCy7染料标记试剂盒Lightning-LinkCy71reaction(upto2mg)AZK0027ACy3.5染料标记试剂盒Lightning-LinkCy3.53reactions(eachupto200ug)AZK0027BCy3.5染料标记试剂盒Lightning-LinkCy3.51reaction(upto2mg)AZK0028AAtto390荧光染料标记试剂盒Lightning-Linkatto3903reactions(eachupto20ug)AZK0028BAtto390荧光染料标记试剂盒Lightning-Linkatto3903reactions(eachupto200ug)AZK0028CAtto390荧光染料标记试剂盒Lightning-Linkatto3901reaction(upto2mg)AZK0029AAtto425荧光染料标记试剂盒Lightning-Linkatto4253reactions(eachupto20ug)AZK0029BAtto425荧光染料标记试剂盒Lightning-Linkatto4253reactions(eachupto200ug)AZK0029CAtto425荧光染料标记试剂盒Lightning-Linkatto4251reaction(upto2mg)AZK0030AAtto465荧光染料标记试剂盒Lightning-Linkatto4653reactions(eachupto20ug)AZK0030BAtto465荧光染料标记试剂盒Lightning-Linkatto4653reactions(eachupto200ug)AZK0030CAtto465荧光染料标记试剂盒Lightning-Linkatto4651reaction(upto2mg)AZK0031AAtto488荧光染料标记试剂盒Lightning-Linkatto4883reactions(eachupto20ug)AZK0031BAtto488荧光染料标记试剂盒Lightning-Linkatto4883reactions(eachupto200ug)AZK0031CAtto488荧光染料标记试剂盒Lightning-Linkatto4881reaction(upto2mg)AZK0032AAtto532荧光染料标记试剂盒Lightning-Linkatto5323reactions(eachupto20ug)AZK0032BAtto532荧光染料标记试剂盒Lightning-Linkatto5323reactions(eachupto200ug)AZK0032CAtto532荧光染料标记试剂盒Lightning-Linkatto5321reaction(upto2mg)AZK0033AAtto565荧光染料标记试剂盒Lightning-Linkatto5653reactions(eachupto20ug)AZK0033BAtto565荧光染料标记试剂盒Lightning-Linkatto5653reactions(eachupto200ug)AZK0033CAtto565荧光染料标记试剂盒Lightning-Linkatto5651reaction(upto2mg)AZK0034AAtto590荧光染料标记试剂盒Lightning-Linkatto5903reactions(eachupto20ug)AZK0034BAtto590荧光染料标记试剂盒Lightning-Linkatto5903reactions(eachupto200ug)AZK0034CAtto590荧光染料标记试剂盒Lightning-Linkatto5901reaction(upto2mg)AZK0035AAtto594荧光染料标记试剂盒Lightning-Linkatto5943reactions(eachupto20ug)AZK0035BAtto594荧光染料标记试剂盒Lightning-Linkatto5943reactions(eachupto200ug)AZK0035CAtto594荧光染料标记试剂盒Lightning-Linkatto5941reaction(upto2mg)AZK0036AAtto633荧光染料标记试剂盒Lightning-Linkatto6333reactions(eachupto20ug)AZK0036BAtto633荧光染料标记试剂盒Lightning-Linkatto6333reactions(eachupto200ug)AZK0036CAtto633荧光染料标记试剂盒Lightning-Linkatto6331reaction(upto2mg)AZK0037AAtto637荧光染料标记试剂盒Lightning-Linkatto6373reactions(eachupto200ug)AZK0037BAtto637荧光染料标记试剂盒Lightning-Linkatto6371reaction(upto2)mgAZK0038AAtto655荧光染料标记试剂盒Lightning-Linkatto6553reactions(eachupto20ug)AZK0038BAtto655荧光染料标记试剂盒Lightning-Linkatto6553reactions(eachupto200ug)AZK0038CAtto655荧光染料标记试剂盒Lightning-Linkatto6551reaction(upto2mg)AZK0039AAtto680荧光染料标记试剂盒Lightning-Linkatto6803reactions(eachupto20ug)AZK0039BAtto680荧光染料标记试剂盒Lightning-Linkatto6803reactions(eachupto200ug)AZK0039CAtto680荧光染料标记试剂盒Lightning-Linkatto6801reaction(upto2mg)AZK0040AAtto700荧光染料标记试剂盒Lightning-Linkatto7003reactions(eachupto20ug)AZK0040BAtto700荧光染料标记试剂盒Lightning-Linkatto7003reactions(eachupto200ug)AZK0040CAtto700荧光染料标记试剂盒Lightning-Linkatto7001reaction(upto2mg)AZK0041AFluoProbesDyes547H荧光探针染料标记试剂盒Lightning-LinkFluoProbes547H3reactions(eachupto20ug)AZK0041BFluoProbesDyes547H荧光探针染料标记试剂盒Lightning-LinkFluoProbes547H3reactions(eachupto200ug)AZK0041CFluoProbesDyes547H荧光探针染料标记试剂盒Lightning-LinkFluoProbes547H1reaction(upto2mg)AZK0042AFluoProbesDyes594荧光探针染料标记试剂盒Lightning-LinkFluoProbes5943reactions(eachupto20ug)AZK0042BFluoProbesDyes594荧光探针染料标记试剂盒Lightning-LinkFluoProbes5943reactions(eachupto200ug)AZK0042CFluoProbesDyes594荧光探针染料标记试剂盒Lightning-LinkFluoProbes5941reaction(upto2mg)AZK0043AFluoProbesDyes647H荧光探针染料标记试剂盒Lightning-LinkFluoProbes647H3reactions(eachupto20ug)AZK0043BFluoProbesDyes647H荧光探针染料标记试剂盒Lightning-LinkFluoProbes647H3reactions(eachupto200ug)AZK0043CFluoProbesDyes647H荧光探针染料标记试剂盒Lightning-LinkFluoProbes647H1reaction(upto2mg)AZK0044AFluoProbesDyes682荧光探针染料标记试剂盒Lightning-LinkFluoProbes6823reactions(eachupto20ug)AZK0044BFluoProbesDyes682荧光探针染料标记试剂盒Lightning-LinkFluoProbes6823reactions(eachupto200ug)AZK0044CFluoProbesDyes682荧光探针染料标记试剂盒Lightning-LinkFluoProbes6821reaction(upto2mg)AZK0045AFluoProbesDyes752荧光探针染料标记试剂盒Lightning-LinkFluoProbes7523reactions(eachupto20ug)AZK0045BFluoProbesDyes752荧光探针染料标记试剂盒Lightning-LinkFluoProbes7523reactions(eachupto200ug)AZK0045CFluoProbesDyes752荧光探针染料标记试剂盒Lightning-LinkFluoProbes7521reaction(upto2mg)AZK0046APE/Cy5串联染料Lightning-LinkPE/Cy53x10ugAZK0046BPE/Cy5串联染料Lightning-LinkPE/Cy51x100ugAZK0046CPE/Cy5串联染料Lightning-LinkPE/Cy53x100ugAZK0046DPE/Cy5串联染料Lightning-LinkPE/Cy51x1mgAZK0047APE/Cy5.5串联染料Lightning-LinkPE/Cy5.53x10ugAZK0047BPE/Cy5.5串联染料Lightning-LinkPE/Cy5.51x100ugAZK0047CPE/Cy5.5串联染料Lightning-LinkPE/Cy5.53x100ugAZK0047DPE/Cy5.5串联染料Lightning-LinkPE/Cy5.51x1mgAZK0048APE/Cy7串联染料Lightning-LinkPE/Cy73x10ugAZK0048BPE/Cy7串联染料Lightning-LinkPE/Cy71x100ugAZK0048CPE/Cy7串联染料Lightning-LinkPE/Cy73x100ugAZK0048DPE/Cy7串联染料Lightning-LinkPE/Cy71x1mgAZK0049APE/Cy5.5串联染料Lightning-LinkPerCP/Cy5.53x10ugAZK0049BPE/Cy5.5串联染料Lightning-LinkPerCP/Cy5.51x100ugAZK0050APE/TexasRed串联染料Lightning-LinkPE/TexasRed3x10ugAZK0050BPE/TexasRed串联染料Lightning-LinkPE/TexasRed1x100ugAZK0050CPE/TexasRed串联染料Lightning-LinkPE/TexasRed3x100ugAZK0050DPE/TexasRed串联染料Lightning-LinkPE/TexasRed1x1mgAZK0051APE/Atto594串联染料Lightning-Link?PE/Atto5943x10ugAZK0051BPE/Atto594串联染料Lightning-Link?PE/Atto5941x100ugAZK0051CPE/Atto594串联染料Lightning-Link?PE/Atto5943x100ugAZK0051DPE/Atto594串联染料Lightning-Link?PE/Atto5941x1mgAZK0052APerCP/Cy5.5串联染料Lightning-LinkPerCP/Cy5.53x100ugAZK0052BPerCP/Cy5.5串联染料Lightning-LinkPerCP/Cy5.51x1mgAZK0052CPerCP/Cy5.5串联染料Lightning-LinkAPC/Cy5.53x10ugAZK0052DPerCP/Cy5.5串联染料Lightning-LinkAPC/Cy5.51x100ugAZK0052EPerCP/Cy5.5串联染料Lightning-LinkAPC/Cy5.53x100ugAZK0052FPerCP/Cy5.5串联染料Lightning-LinkAPC/Cy5.51x1mgAZK0053ALightning-LinkAPC/Cy7串联染料Lightning-LinkAPC/Cy73x10ugAZK0053BLightning-LinkAPC/Cy7串联染料Lightning-LinkAPC/Cy71x100ugAZK0053CLightning-LinkAPC/Cy7串联染料Lightning-LinkAPC/Cy73x100ugAZK0053DLightning-LinkAPC/Cy7串联染料Lightning-LinkAPC/Cy71x1mgAZK0054A生物素化结合试剂盒typeALightning-LinkBiotinconjugationkittypeA3reactions(upto20μgAb)AZK0054B生物素化结合试剂盒typeALightning-LinkBiotinconjugationkittypeA3reactions(upto200μgAb)AZK0054C生物素化结合试剂盒typeALightning-LinkBiotinconjugationkittypeA1reaction(upto2mgAb)AZK0055A生物素化结合试剂盒typeBLightning-LinkBiotinconjugationkittypeB3reactions(upto20μgAb)AZK0055B生物素化结合试剂盒typeBLightning-LinkBiotinconjugationkittypeB3reactions(upto200μgAb)AZK0055C生物素化结合试剂盒typeBLightning-LinkBiotinconjugationkittypeB1reaction(upto2mgAb)AZK0056A链亲和素标记试剂盒Lightning-LinkStreptavidinconjugationkit3x10ugStreptavidinAZK0056B链亲和素标记试剂盒Lightning-LinkStreptavidinconjugationkit3x100ugStreptavidinAZK0056C链亲和素标记试剂盒Lightning-LinkStreptavidinconjugationkit1x1mgStreptavidinAZK0057A亲和素标记试剂盒Lightning-LinkAvidinconjugationkit3x10ugAvidinAZK0057B亲和素标记试剂盒Lightning-LinkAvidinconjugationkit3x100ugAvidinAZK0057C亲和素标记试剂盒Lightning-LinkAvidinconjugationkit1x1mgAvidinAZK0058A快速荧光素标记Lightning-Link?RapidFluorescein3xAblabelings(each10-20ugscale)AZK0058B快速荧光素标记Lightning-Link?RapidFluorescein3xAblabelings(each100-200ugscale)AZK0058C快速荧光素标记Lightning-Link?RapidFluorescein1xAblabeling(1scale)AZK0059A快速Cy3标记Lightning-Link?RapidCy33xAblabelings(each10-20ugscale)AZK0059B快速Cy3标记Lightning-Link?RapidCy33xAblabelings(each100-200ugscale)AZK0059C快速Cy3标记Lightning-Link?RapidCy31xAblabeling(1scale)AZK0060A快速Cy5标记Lightning-Link?RapidCy53xAblabelings(each10-20ugscale)AZK0060B快速Cy5标记Lightning-Link?RapidCy53xAblabelings(each100-200ugscale)AZK0060C快速Cy5标记Lightning-Link?RapidCy51xAblabeling(1scale)AZK0061A快速Atto488标记Lightning-Link?RapidAtto4883xAblabelings(each10-20ugscale)AZK0061B快速Atto488标记Lightning-Link?RapidAtto4883xAblabelings(each100-200ugscale)AZK0061C快速Atto488标记Lightning-Link?RapidAtto4881xAblabeling(1scale)AZK0062A快速Atto633标记Lightning-Link?RapidAtto6333xAblabelings(each10-20ugscale)AZK0062B快速Atto633标记Lightning-Link?RapidAtto6333xAblabelings(each100-200ugscale)AZK0062C快速Atto633标记Lightning-Link?RapidAtto6331xAblabeling(1scale)AZK0063A快速Atto565标记Lightning-Link?RapidAtto5653xAblabelings(each10-20ugscale)AZK0063B快速Atto565标记Lightning-Link?RapidAtto5653xAblabelings(each100-200ugscale)AZK0063C快速Atto565标记Lightning-Link?RapidAtto5651xAblabeling(1scale)AZK0064A快速Atto594标记Lightning-Link?RapidAtto5943xAblabelings(each10-20ugscale)AZK0064B快速Atto594标记Lightning-Link?RapidAtto5943xAblabelings(each100-200ugscale)AZK0064C快速Atto594标记Lightning-Link?RapidAtto5941xAblabeling(1scale)AZK0065A快速罗丹明标记Lightning-Link?RapidRhodamine3xAblabelings(each10-20ugscale)AZK0065B快速罗丹明标记Lightning-Link?RapidRhodamine3xAblabelings(each100-200ugscale)AZK0065C快速罗丹明标记Lightning-Link?RapidRhodamine1xAblabeling(1scale)AZK0066A快速Atto390标记Lightning-Link?RapidAtto3903xAblabelings(each10-20ugscale)AZK0066B快速Atto390标记Lightning-Link?RapidAtto3903xAblabelings(each100-200ugscale)AZK0066C快速Atto390标记Lightning-Link?RapidAtto3901xAblabeling(1scale)AZK0067A快速DyLight350标记Lightning-Link?RapidDyLight3503xAblabelings(each10-20ugscale)AZK0068A快速DyLight350素标记Lightning-Link?RapidDyLight3503xAblabelings(each100-200ugscale)AZK0069A快速DyLight350标记Lightning-Link?RapidDyLight3501xAblabeling(1scale)AZK0070A快速DyLight405标记Lightning-Link?RapidDyLight4053xAblabelings(each10-20ugscale)AZK0070B快速DyLight405标记Lightning-Link?RapidDyLight4053xAblabelings(each100-200ugscale)AZK0070C快速DyLight405标记Lightning-Link?RapidDyLight405
其检测原理为:在正常的活细胞中,磷脂酰丝氨酸(phosphotidylserine,PS)位于细胞膜的内侧,但在早期凋亡的细胞中,PS 从细胞膜的内侧翻转到细胞膜的表面,暴露在细胞外环境中。Annexin-Ⅴ(膜联蛋白-V)是一种分子量为35-36KD的Ca2+ 依赖性磷脂结合蛋白,能与PS高亲和力结合。可通过细胞外侧暴露的磷脂酰丝氨酸与凋亡早期细胞的胞膜结合。
操作步骤:
1.从室温平衡20min后的铝箔袋中取出所需板条,剩余板条用自封袋密封放回4℃。
2.设置标准品孔和样本孔,标准品孔各加不同浓度的标准品50μL;
3.样本孔中加入待测样本50μL;空白孔不加。
4.除空白孔外,标准品孔和样本孔中每孔加入辣根过氧化物酶(HRP)标记的检测抗体100μL,用封板膜封住反应孔,37℃水浴锅或恒温箱温育60min。
5.弃去液体,吸水纸上拍干,每孔加满洗涤液(350μL),静置1min,甩去洗涤液,吸水纸上拍干,如此重复洗板5次(也可用洗板机洗板)。
6.每孔加入底物A、B各50μL,37℃避光孵育15min。
7.每孔加入终止液50μL,15min内,在450nm波长处测定各孔的OD值。
不知道发在这里合适不,实在是求助无门啊!版主手下留情。

