货号：	786-313-5reactions
数量：	大量
英文名：	HOOK™HRPPLUSLabelingkit
规格：	5reactions

Description:TheHOOK™HRPPLUSlabelingkitisahighefficiencyenzymelabelingkitfortaggingproteinswithhorseradishperoxidaseenzyme.ThiskithasanactivatedHRPthatcoupleswithhighefficiency(>90%)tothenumerousaminegroupsofproteinsandissuperiortothecommonlyusedglutaraldehydecouplingchemistry.ThiskitusesHOOK™HRPPLUS,whichisHRPthathasbeenactivatedbytheadditionofreactivealdehydes.Thealdehydegroupsreactspontaneouslyandathighefficiencywithprimaryamines,locatedattheN-terminusofproteinsorinlysineresidues,toformintermediateSchiffBasecomplexes.These,inturn,areselectivelyreducedbythesuppliedreductionagent.Followingquenchingofthereactiontheproteinislinkedtothehorseradishperoxidaseenzymebystableaminelinkage.Thelabeledprotein,orantibody,cannowbeusedforimmunoblotting,ELISAandhistochemicaltechniques.ThiskitissuppliedwithJAW™DryBufferpacksforconvenientPBSandCarbonate/Bicarbonatebufferpreparation.Features:Activityis120-200units/mg;Reactswithprimaryaminestoformcovalentaminebonds;Stablefor>12monthsat-20°C;Highconsistency，Store:-15～-20℃