MutaGEL ACE PCR Assay Kit
MutaGEL ACE PCR Assay manufactured in Germany by Immundiagnostik
Size: 24 Samples
Method: PCR (allele-specific)
Sample Type: DNA (e.g.whole blood, cheek swab)
Sample Size: 200 µL
Alternative Names: Angiotensin-convertin Enzyme PCR Assay, Human ACE PCR Assay
For Research Use Only
Control Included
Assay Background
The angiotensin-converting enzyme (ACE) is the most important regulator of the renin/ angiotensin/ aldosterone system (RAAS). The enzyme converts angiotensin 1 into the physiologically active form angiotensin 2. Latter one unfolds its vasoconstrictive effect directly into the organs (heart, kidney, vessels). The insertion/ deletion polymorphism in the ACE gene is associated immediately at the circulating ACE level. Therefore the I / D -polymorphism influences the risk for cardiovascular complications.
In contrary, the “S”-mutation (Glu to Val in codon 246 of exon 3) has protective properties: S-homozygotes and also compound heterozygotes with the Z-mutation (SZ) does not cause liver diseases. The enzyme product of the “S”-mutation has a short half-life and consequently a decreased serum activity. Hereditary antitrypsin deficiency with organ-defects is characterized by lung emphysema or chronic hepatitis (resp. liver cirrhosis or hepatocellular carcinoma) and is in Europe with a frequency of 1: 2-5000 the main cause for the mentioned hereditary liver diseases by children.
Storage and Stability
Storage at < -18°c.="" the="" reagents="" are="" stable="" in="" the="" unopened="" micro="" tubes="" until="" the="" expiration="" date="" indicated.="" don"t="" thaw="" out="" the="" content="" of="" the="" “ace="" positive="" control="" dna”="" more="" than="" twice.="" if="" necessary,="" make="" suitable="" aliquots.="" before="" use:="" spin="" tubes="" briefly="" before="" opening="" (contents="" may="" become="" dispersed="" during="" shipment).="">
Related Products
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MutaGEL MTHFR PCR Assay Kit
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Restrictions
The PCR results for positive control in DNA fragments of indicated length and for the samples at least one of both amplification products must be detactable. If this is not the case, the sample must be tested a second time or the complete analysis must be repeated with freshly isolated DNA. If there are no positive control DNA fragments present, the amplification was incorrect and the chosen PCR conditions have to been proven/ corrected.
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为了使供输血用的血液能保存一定时间，加入适当的抗凝剂，并尽量防止在保存期发生变质而用的液体。为了防止因糖的消失引起活力降低，尚加入葡萄糖。ACD液（含柠檬酸三钠、柠檬酸、葡萄糖）一般用于血库。添加ACD的血液放在4—6℃条件下保存，可保存三周。另外为防止柠檬酸盐的过量可用加磷酸钠的CPD液。此外还设计了加乳酸钠的ACDL液。如果加入甘油等防冻剂则可长时间冷冻保存红细胞。
我搜索了一下网络上的，只找到说要保存在酸性下，但是没有具体的配方。
目前选择这个都是北京博凌科为生物科技有限公司的
小白一枚，现在要构建紫杉醇细胞耐药株。但是我们实验室紫杉醇是医用注射液，浓度为6mg/ml。这个浓度太大了，工作液浓度都是几十ng/ml。想问一下各位大神该如何配置成浓度相对低一点的储存液呢，有效期可以有多久呢？
目前正在从新鲜病人肿瘤组织标本中提取细胞悬液进行阳性细胞的筛选，但由于实验室距离医院有3个小时的车程，请问什么储存方法或者储存液能短期保证肿瘤组织中细胞完整性？谢谢
