MutaGEL ACE PCR Assay Kit
MutaGEL ACE PCR Assay manufactured in Germany by Immundiagnostik
Size: 24 Samples
Method: PCR (allele-specific)
Sample Type: DNA (e.g.whole blood, cheek swab)
Sample Size: 200 µL
Alternative Names: Angiotensin-convertin Enzyme PCR Assay, Human ACE PCR Assay
For Research Use Only
Control Included
Assay Background
The angiotensin-converting enzyme (ACE) is the most important regulator of the renin/ angiotensin/ aldosterone system (RAAS). The enzyme converts angiotensin 1 into the physiologically active form angiotensin 2. Latter one unfolds its vasoconstrictive effect directly into the organs (heart, kidney, vessels). The insertion/ deletion polymorphism in the ACE gene is associated immediately at the circulating ACE level. Therefore the I / D -polymorphism influences the risk for cardiovascular complications.
In contrary, the “S”-mutation (Glu to Val in codon 246 of exon 3) has protective properties: S-homozygotes and also compound heterozygotes with the Z-mutation (SZ) does not cause liver diseases. The enzyme product of the “S”-mutation has a short half-life and consequently a decreased serum activity. Hereditary antitrypsin deficiency with organ-defects is characterized by lung emphysema or chronic hepatitis (resp. liver cirrhosis or hepatocellular carcinoma) and is in Europe with a frequency of 1: 2-5000 the main cause for the mentioned hereditary liver diseases by children.
Storage and Stability
Storage at < -18°c.="" the="" reagents="" are="" stable="" in="" the="" unopened="" micro="" tubes="" until="" the="" expiration="" date="" indicated.="" don"t="" thaw="" out="" the="" content="" of="" the="" “ace="" positive="" control="" dna”="" more="" than="" twice.="" if="" necessary,="" make="" suitable="" aliquots.="" before="" use:="" spin="" tubes="" briefly="" before="" opening="" (contents="" may="" become="" dispersed="" during="" shipment).="">
Related Products
MutaGEL Apolipoprotein B 100 PCR Assay
MutaGEL MTHFR PCR Assay Kit
MutaGEL Prothrombin PCR Assay
Restrictions
The PCR results for positive control in DNA fragments of indicated length and for the samples at least one of both amplification products must be detactable. If this is not the case, the sample must be tested a second time or the complete analysis must be repeated with freshly isolated DNA. If there are no positive control DNA fragments present, the amplification was incorrect and the chosen PCR conditions have to been proven/ corrected.
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我搜索了一下网络上的，只找到说要保存在酸性下，但是没有具体的配方。
如果需要储液箱，那储液箱和微型定量泵之间用什么连接？是用管道吗？
在官方网上查到的可用产品信息如下。Leupeptin用水溶解，工作液有效浓度是10-100uM.我认为这个数值太大,我怎么算也不知道该加多少量..
PMSF用5%乙醇溶解，有效浓度没有说。怎样配,有效浓度应该是多少?
使用过的同学,帮忙讲几句.
小白一枚，现在要构建紫杉醇细胞耐药株。但是我们实验室紫杉醇是医用注射液，浓度为6mg/ml。这个浓度太大了，工作液浓度都是几十ng/ml。想问一下各位大神该如何配置成浓度相对低一点的储存液呢，有效期可以有多久呢？
生产单位 天津百利鑫生物科技有限公司
地址
邮编
产品名称 细胞保存液
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从以上药监局已注册的产品信息看，属于第1类
