MutaPLATE Lactase PCR Assay Kit
MutaPLATE Lactase PCR Assay manufactured in Germany by Immundiagnostik
Size: 32 tests
Method: PCR
Sample Type: DNA (e.g.whole blood, cheek swab)
Sample Size: 200 µL
For Research Use Only
Assay Principle
The Eagle Biosciences MutaPLATE® Lactase real-time PCR Kit contains specific primers and additional material for the detection of the T/C (-13910) polymorphism in the regulatory region of the lactase-phlorizin-hydrolase gene. The variable area of the regulatory region from lactase gene is amplified by PCR using genomic DNA-template. The specific primers used in the kit flank the variable area of the lactase gene (LCT) and generate an amplification of 222 bp.
The standard PCR contains also sequence-specific oligonucleotides marked with fluorescence dye. This specific hybridization probe binds within the amplification product including the single nucleotide polymorphism (SNP) of target-DNA. Due to this, a fluorescence signal is generated (after excitation with 530 nm or if limited by an instrument with 470 nm) and detected at 610 nm through the optical unit of the real-time PCR instrument.
Genotyping is performed by subsequent melting curve analysis of arisen amplificates leading to unequivocal identification of C/C-genotype associated with lactose intolerance and respectively the clinical unobtrusive CT- and T/T-variants. This is due to the different melting points of the complexes formed by DNA template and “SNP-probes”. The included “SNP-probe” is 100% homologous to the C-allele. Therefore the hybridization probe needs a higher temperature for complex-dissociation from C-allele than from the T-allele (containing a mismatch destabilizing the complex). Consequently, samples with heterozygous genotype generate both peaks at different temperatures during the melting curve analysis.
Related Products
MutaPLATE HLA-DQ2+8 (TM) PCR Assay Kit
MutaPLATE® Lactase TAQ-man PCR Assay Kit
MutaGEL® Lactase PCR Assay
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为了使供输血用的血液能保存一定时间，加入适当的抗凝剂，并尽量防止在保存期发生变质而用的液体。为了防止因糖的消失引起活力降低，尚加入葡萄糖。ACD液（含柠檬酸三钠、柠檬酸、葡萄糖）一般用于血库。添加ACD的血液放在4—6℃条件下保存，可保存三周。另外为防止柠檬酸盐的过量可用加磷酸钠的CPD液。此外还设计了加乳酸钠的ACDL液。如果加入甘油等防冻剂则可长时间冷冻保存红细胞。
目前选择这个都是北京博凌科为生物科技有限公司的
目前正在从新鲜病人肿瘤组织标本中提取细胞悬液进行阳性细胞的筛选，但由于实验室距离医院有3个小时的车程，请问什么储存方法或者储存液能短期保证肿瘤组织中细胞完整性？谢谢
