The Rabbit TrueBlot® Western Blot Kit contains the critical supporting reagents, buffers, and substrates for immunoprecipitation and Western blotting of samples using TrueBlot second step immunoblotting reagents in conjunction with your own primary IP antibody and primary (Rabbit IgG) Western blotting antibody. TrueBlot technology enables unhindered detection of protein bands of interest which would otherwise be obscured by the presence of reduced & denatured heavy and light chain immunoglobulin in the blot (as detected by the conventional immunoblotting HRP anti-rabbit IgG reagent). Rabbit IgG TrueBlot® is the unique horseradish peroxidase conjugated anti-rabbit IgG immunoblotting second step reagent which enables detection of immunoblotted target protein bands, without hindrance by interfering immunoglobulin heavy and light chains from your IP antibody. Use it in place of your usual HRP anti-rabbit IgG immunoblotting second step reagent. It is easy to generate publication-quality IP/WB data with Rabbit IgGTrueBlot®. Rabbit IgG TrueBlot is ideal for use in protocols involving immunoblotting of immunoprecipitated proteins. TrueBlot preferentially detects the non-reduced form of rabbit IgG over the reduced, SDS-denatured form of IgG. When the immunoprecipitate is fully reduced immediately prior to SDS-gel electrophoresis, reactivity of Rabbit IgG TrueBlot with the 55 kDa heavy chains and the 23 kDa light chains of the immunoprecipitating antibody is minimized thereby eliminating interference by the heavy and light chains of the immunoprecipitating antibody in IP/immunoblotting applications. Applications include studies examining post-translational modification (e.g., phosphorylation or acetylation) or protein-protein interactions. Rabbit IgG TrueBlot may also be used for detection in immunoblotting assays that do not employ immunoprecipitation.
| Keywords: | TrueBlot®, IP/WB |
| Supplier: | Rockland Immunochemicals |
| Supplier-Nr: | 88-8886-31 |
Properties
| Application: | IP, WB |
| Antibody Type: | Monoclonal |
| Clone: | eB182 |
| Host: | Mouse |
| Reactivity: | Rabbit |
Database Information
Handling & Safety
| Storage: | +4°C (do not freeze!) |
| Shipping: | +4°C (International: +4°C) |
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如果需要储液箱，那储液箱和微型定量泵之间用什么连接？是用管道吗？
在官方网上查到的可用产品信息如下。Leupeptin用水溶解，工作液有效浓度是10-100uM.我认为这个数值太大,我怎么算也不知道该加多少量..
PMSF用5%乙醇溶解，有效浓度没有说。怎样配,有效浓度应该是多少?
使用过的同学,帮忙讲几句.
类医疗器械？谢谢！
我搜索了一下网络上的，只找到说要保存在酸性下，但是没有具体的配方。
生产单位 天津百利鑫生物科技有限公司
地址
邮编
产品名称 细胞保存液
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从以上药监局已注册的产品信息看，属于第1类
小白一枚，现在要构建紫杉醇细胞耐药株。但是我们实验室紫杉醇是医用注射液，浓度为6mg/ml。这个浓度太大了，工作液浓度都是几十ng/ml。想问一下各位大神该如何配置成浓度相对低一点的储存液呢，有效期可以有多久呢？
