Isotype: Murine IgG1k
Immunogen: Recombinant human CD279
INFORMATION: Human CD279 (PD-1, Programmed death 1) is a 55 kd Ig superfamily member with sililarity to CD28 and CD152(CTLA-4). It is expressed on activated T and B and myeloid cells, and engagement by its ligands PD-L1 (CD274, B7-H1) or PD-L2 (B7-DC) can inhibit proliferation and cytokine expression. In mice blockade of PD-1 ligand interaction has been used to augment T cell anti cancer responses (3,4,5).
Anti-CD279 antibody clone ANC4H6 binds to CD279 present on PHA activated PBMC in FACS, and binds to recombinant CD279 in EIA. Pre incubation of recombinant CD279-coated plates with ANC4H6 blocks binding of biotinylated recombinant CD273 in EIA.
References:
1) Ishida et al. (1992) EMBO J 11(11):3887-95.
2) Carreno BM, M Collins, et al. (2001) Annu Rev Immunol 20:29.
3) MA Curran, Allison JP, et al. (2010) PNAS 107(9): 4275-80.
4) Mangsbo SM, TH Totterman, et al. (2010) J Immunotherapy 33(3):225.
5) Hirano F, L Chen, et al. (2005) Canc Res 65: 1089.
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目前正在从新鲜病人肿瘤组织标本中提取细胞悬液进行阳性细胞的筛选，但由于实验室距离医院有3个小时的车程，请问什么储存方法或者储存液能短期保证肿瘤组织中细胞完整性？谢谢
小白一枚，现在要构建紫杉醇细胞耐药株。但是我们实验室紫杉醇是医用注射液，浓度为6mg/ml。这个浓度太大了，工作液浓度都是几十ng/ml。想问一下各位大神该如何配置成浓度相对低一点的储存液呢，有效期可以有多久呢？
目前选择这个都是北京博凌科为生物科技有限公司的
类医疗器械？谢谢！
