Glucocorticoid induced Tumor Necrosis Factor receptor family related gene (GITR, AITR) is a type 1 228 amino acid transmembrane protein that has been designated TNFRSF18. GITR is expressed mainly by CD4+ CD25+ T lymphocytes. Its engagement by antibodies or its ligand (GITRL) induces a costimulatory signal which can break T cell suppression (2). GITR has been implicated as a regulatory mechanism in anti-parasitic immune resposes (4), and is involved with Macrophages activation in atherosclerotic plaque formation (3).
Clone ANC5A3 binds to human GITR in EIA and on stimulated cell surface in FACS. Preincubation of the receptor with an excess of this mAb will block binding of recombinant GITR Ligand.
Isotype: Murine IgG3 kappa
Immunogen: Recombinant human GITR, PHA stimulated human PBL
Specificity: Antibody ANC5A3 binds to human GITR in EIA and on cell surface in FACS. Preincubation of the receptor with an excess of this mAb will block binding of recombinant GITR Ligand.
References:
1) Gurney AL, et al. (1999) Curr Biol 9(4): 215-218.
2) Ji HB, et al. (2004 ) J Immunol 172(10): 5823-5827.
3) Kim WJ, WH Lee, et al. (2006) Immunology 119(3): 44-9.
4) Furze RC, Selkirk ME, et al. (2006) Microbes Infect 8 (12-13): 2803-10.
5) Kohm AP, SD Miller, et al. (2004) J Immunol 172: 4686-4690.
6) McHugh, R.S. et al. (2002) Immunity 16:311.
7) Shin, H.H. et al. (2002) FEBS Lett. 514:275.
8). Shimizu, J. et al. (2002) Nature Immunol. 3:135.
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目前选择这个都是北京博凌科为生物科技有限公司的
在官方网上查到的可用产品信息如下。Leupeptin用水溶解，工作液有效浓度是10-100uM.我认为这个数值太大,我怎么算也不知道该加多少量..
PMSF用5%乙醇溶解，有效浓度没有说。怎样配,有效浓度应该是多少?
使用过的同学,帮忙讲几句.
