Glucocorticoid induced Tumor Necrosis Factor receptor family related gene (GITR, AITR) is a type 1 228 amino acid transmembrane protein that has been designated TNFRSF18. GITR is expressed mainly by CD4+ CD25+ T lymphocytes. Its engagement by antibodies or its ligand (GITRL) induces a costimulatory signal which can break T cell suppression (2). GITR has been implicated as a regulatory mechanism in anti-parasitic immune resposes (4), and is involved with Macrophages activation in atherosclerotic plaque formation (3).
Clone ANC5A3 binds to human GITR in EIA and on stimulated cell surface in FACS. Preincubation of the receptor with an excess of this mAb will block binding of recombinant GITR Ligand.
Isotype: Murine IgG3 kappa
Immunogen: Recombinant human GITR, PHA stimulated human PBL
Specificity: Antibody ANC5A3 binds to human GITR in EIA and on cell surface in FACS. Preincubation of the receptor with an excess of this mAb will block binding of recombinant GITR Ligand.
References:
1) Gurney AL, et al. (1999) Curr Biol 9(4): 215-218.
2) Ji HB, et al. (2004 ) J Immunol 172(10): 5823-5827.
3) Kim WJ, WH Lee, et al. (2006) Immunology 119(3): 44-9.
4) Furze RC, Selkirk ME, et al. (2006) Microbes Infect 8 (12-13): 2803-10.
5) Kohm AP, SD Miller, et al. (2004) J Immunol 172: 4686-4690.
6) McHugh, R.S. et al. (2002) Immunity 16:311.
7) Shin, H.H. et al. (2002) FEBS Lett. 514:275.
8). Shimizu, J. et al. (2002) Nature Immunol. 3:135.
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
为了使供输血用的血液能保存一定时间，加入适当的抗凝剂，并尽量防止在保存期发生变质而用的液体。为了防止因糖的消失引起活力降低，尚加入葡萄糖。ACD液（含柠檬酸三钠、柠檬酸、葡萄糖）一般用于血库。添加ACD的血液放在4—6℃条件下保存，可保存三周。另外为防止柠檬酸盐的过量可用加磷酸钠的CPD液。此外还设计了加乳酸钠的ACDL液。如果加入甘油等防冻剂则可长时间冷冻保存红细胞。
目前选择这个都是北京博凌科为生物科技有限公司的
目前正在从新鲜病人肿瘤组织标本中提取细胞悬液进行阳性细胞的筛选，但由于实验室距离医院有3个小时的车程，请问什么储存方法或者储存液能短期保证肿瘤组织中细胞完整性？谢谢
