Human CD28 is an important costimulatory molecule found on all CD4+ T cells and on about half of the CD8+T cells. T cell activities attributed to CD28 include prevention of anergy, induction of cytokine gene transcription, stabilization of cytokine mRNAs and activation of CD8+ cytotoxic T lymphocytes. The ligands for CD28 identified as CD80 (B7-1) and CD86 (B7-2), are immunoglobulin superfamily monomeric transmembrane glycoprotein of 60 kd and 80 kd, respectively. Monovalencyof the CD28 receptor for its natural ligands is essential to providecostimulation without inducing responses in the absense of TCRengagement (5)
Molecular Structure:A soluble fusion protein consisting of the mature extracellular (134aa) domain of human CD28: nkilvkqspmlvaydnavnlsckysynlfsrefraslhkgldsavevcvvygnysqqlqvysktgfncdgklgnesvtfylqnlyvnqtdiyfckievmypppyldneksngtiihvkgkhlcpsplfpgpskp
+linker: gt
fused to murine IgG2a Fc + hinge (233aa): eprgptikpcppckcpapnllggpsvfifppkikdvlmislspivtcvvvdvseddpdvqiswfvnnvevhtaqtqthredynstlrvvsalpiqhqdwmsgkefkckvnnkdlpapiertiskpkgsvrapqvyvlpppeeemtkkqvtltcmvtdfmpediyvewtnngktelnykntepvldsdgsyfmysklrvekknwvernsyscsvvheglhnhhttksfsrtpg
predicted (non glycosylated ) monomeric molecular weight: 41.8 kd
predicted extinction coeficient: 1.13 OD for a 1mg/ml solution
Transfectant Cell Line: CHO
Functional Application: Recombinant human CD28 -muIg fusion protein blocks binding of anti-human CD28 to human tumor cells. In combination with IFN-gamma, CD28-muIg can stimulate dendritic cells in when a crosslinking agent is included (6).
References:
1. M.K. Jenkins & J.G. Johnson, (1993) Curr Opin Immunol 5: 361-367.
2. 61st Forum in Immunology, (1995) Res Immunol 146: 127-205.
3.I. Kariv, A. Truneh & R.W. Sweet, (1996) J Immunol 157: 29-38.
4. R.J. Peach, Linsley P.S., et al. J Biol Chem (1995) 270(36): 21181-21187.
5) K M Dennehy, T Hunig, et al, (2006) J Immunol 176: 5725-5729.
6. D H Munn, M D Sharma, A L Mellor (2004) J immunol 172(7): 4100-10. PMID: 15034022
ebiomall.com
>
>
>
>
>
>
>
>
>
>
类医疗器械？谢谢！
小白一枚，现在要构建紫杉醇细胞耐药株。但是我们实验室紫杉醇是医用注射液，浓度为6mg/ml。这个浓度太大了，工作液浓度都是几十ng/ml。想问一下各位大神该如何配置成浓度相对低一点的储存液呢，有效期可以有多久呢？
我搜索了一下网络上的，只找到说要保存在酸性下，但是没有具体的配方。
生产单位 天津百利鑫生物科技有限公司
地址
邮编
产品名称 细胞保存液
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
从以上药监局已注册的产品信息看，属于第1类
