ISOTYPE: Mouse IgG1k
INFORMATION: The p75 low affinity human Nerve Growth Factor receptor CD271(NGRF, TNFRSF16) is expressed on a variety of tissue types including a subset of splenic and nodal lymphocytes. Presence of this receptor supports uptake of intracellular calcium, but not mobilization (3). CD271 is a stem cell marker for hematopoietic(5,6,7), stromal (8) as well as cancer cells (9,10). CD271 has been reported to inhibit differentiation of mesenchymal cells into various lineages(11).
Antibody ANC271/3D7 binds to recombinant CD271-muIg (cat# 527-020) in EIA, and to endogenous CD271 on cell surface of human Reh cells.
REFERENCES:
1) Brodie C, E W Gelfand (1992) Journal of Immunology. 148(11): 3492-3497.
2) Fantini F, O. Johansson (1992) Journal of Investigative Dermatology. 99(6): 734-742.
3) Jiang H, G Gurogg, et al.(1999) J Biol Chem 274(37): 26209-16.
4) Khwaja F, D Djakiew, et al (2004) Cancer Res 64(17): 6207-13.
5) Flores-Torales E, Cuneo-Pareto S, et al. (2010) Folia Histochem Cytobiol. 48(4):682-6.
6) Kuçi S, Bader P, et al. (2010) Haematologica. 95(4):651-9.
7) Hermida-Gómez T, Blanco FJ, et al. (2011) Tissue Eng Part A. 17(7-8):1169-79.
8) Jones E, McGonagle D, et al. (2010) Arthritis Rheum. 62(7):1944-54.
9) Boiko AD, Weissman IL. Et al. (2010) Nature 466(7302):133-7.
10) Civenni G, Sommer L, et al. (2011) Cancer Res. 71(8):3098-109.
11) Mikami Y, Honda MJ, et al. (2011) Stem Cells Dev. 20(5):901-13.
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为了使供输血用的血液能保存一定时间，加入适当的抗凝剂，并尽量防止在保存期发生变质而用的液体。为了防止因糖的消失引起活力降低，尚加入葡萄糖。ACD液（含柠檬酸三钠、柠檬酸、葡萄糖）一般用于血库。添加ACD的血液放在4—6℃条件下保存，可保存三周。另外为防止柠檬酸盐的过量可用加磷酸钠的CPD液。此外还设计了加乳酸钠的ACDL液。如果加入甘油等防冻剂则可长时间冷冻保存红细胞。
目前选择这个都是北京博凌科为生物科技有限公司的
目前正在从新鲜病人肿瘤组织标本中提取细胞悬液进行阳性细胞的筛选，但由于实验室距离医院有3个小时的车程，请问什么储存方法或者储存液能短期保证肿瘤组织中细胞完整性？谢谢
