Human Lymphotoxin beta receptor (LTbR, TNFSF3) is a member of the TNF receptor superfamily with similarilty to CD120a(TNFR1) and CD120b(TNFR2). It is a expressed mainly on non lymphoid tissues(1) and appears to have an important role in secondary lymphoid organ development(5). Suppression of LTbR signaling can alleviate autoimmunity(2) or exasperate mycobacterial infection(6). Its ligands include Ltab 2 and LIGHT. In mice, LTbR signaling is important for development and function of HEV(2), Dendritic cells(3), and Mast cells(4).
Molecular Structure: A soluble fusion protein consisting of 10 residual amino acids from murine CD8 alpha leader sequence (KPQAPELRGS), followed by the mature extracellular (192aa) domain of human LTbR (SQPQAVP….PLEPLPP) fused to (233aa) murine IgG2a Fc (EPRGPTI….FSRTPGK). Predicted non glycosylated molecular weight of 48.9 kd.
Transfectant Cell Line: CHO
Functional Application: Recombinant human LTbR-muIg fusion protein binds to recombinant human LIGHT in EIA.
References:
(1)T W Mak, M E Saunders eds. The Immune Response 2006 Elsevier Academic Press p 499.
(2) J L Browning, R.A. Fava, et al. Immunity (2005) 23(5): 539-50.
(3)Y Wang, Y Fu, et al. J Immunol (2005) 175: 6997-7002.
(4)P Stopfer, T Hehlgans, et al. J Immunol (2004) 172: 7459-7465.
(5) Gommermann J, JL Browning, et al, Nat Rev Immunol (2003) 3:642-655.
(6) Spahn TW, T Kucharzik, et al. Infection and Immunity (2005) 73(11): 7077-7088.
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为了使供输血用的血液能保存一定时间，加入适当的抗凝剂，并尽量防止在保存期发生变质而用的液体。为了防止因糖的消失引起活力降低，尚加入葡萄糖。ACD液（含柠檬酸三钠、柠檬酸、葡萄糖）一般用于血库。添加ACD的血液放在4—6℃条件下保存，可保存三周。另外为防止柠檬酸盐的过量可用加磷酸钠的CPD液。此外还设计了加乳酸钠的ACDL液。如果加入甘油等防冻剂则可长时间冷冻保存红细胞。
我搜索了一下网络上的，只找到说要保存在酸性下，但是没有具体的配方。
目前选择这个都是北京博凌科为生物科技有限公司的
小白一枚，现在要构建紫杉醇细胞耐药株。但是我们实验室紫杉醇是医用注射液，浓度为6mg/ml。这个浓度太大了，工作液浓度都是几十ng/ml。想问一下各位大神该如何配置成浓度相对低一点的储存液呢，有效期可以有多久呢？
目前正在从新鲜病人肿瘤组织标本中提取细胞悬液进行阳性细胞的筛选，但由于实验室距离医院有3个小时的车程，请问什么储存方法或者储存液能短期保证肿瘤组织中细胞完整性？谢谢
