Product Overview
Background
Urokinase-type Plasminogen Actvator (uPA) is a glycosylated serine protease that is synthesized in endothelial and kidney epithelial cells. There are two forms of uPA, the low molecular weight (LMW ~31 kDa) and high molecular weight (HMW ~55 kDa). The HMW form undergoes autoproteolysis resulting in the LMW plus and 18.5 kDa amino terminal fragment (ATF). It is this ATF that has been shown to inhibit proliferation and invasion of cancer cells by binding to uPA receptors (1).
Assay Principle
This is an ELISA for the quantitative analysis of active uPA levels in biological fluid. This test kit operates on the basis of sandwich ELISA where free active uPA enzyme complexes with PAI-1 and is quantitated with the use of an HRP labeled secondary antibody.Provided is biotinylated PAI-1 bound to avidin coated wells. This allows active tPA present in the standard or unknown, to complex with PAI-1. Inactive or complexed uPA is removed in a subsequent wash step. A primary antibody specific for uPA is then added to each well followed by the HRP conjugated secondary antibody. The bound conjugated secondary antibody is detected by the addition of substrate, which generates an optimal color after 10 minutes. Quantitative test results may be obtained by the measure and comparison of the sample and standard absorbance readings when read with a microplate reader at 450 nm.
Product Documents
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
如果需要储液箱，那储液箱和微型定量泵之间用什么连接？是用管道吗？
在官方网上查到的可用产品信息如下。Leupeptin用水溶解，工作液有效浓度是10-100uM.我认为这个数值太大,我怎么算也不知道该加多少量..
PMSF用5%乙醇溶解，有效浓度没有说。怎样配,有效浓度应该是多少?
使用过的同学,帮忙讲几句.
类医疗器械？谢谢！
我搜索了一下网络上的，只找到说要保存在酸性下，但是没有具体的配方。
生产单位 天津百利鑫生物科技有限公司
地址
邮编
产品名称 细胞保存液
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
从以上药监局已注册的产品信息看，属于第1类
小白一枚，现在要构建紫杉醇细胞耐药株。但是我们实验室紫杉醇是医用注射液，浓度为6mg/ml。这个浓度太大了，工作液浓度都是几十ng/ml。想问一下各位大神该如何配置成浓度相对低一点的储存液呢，有效期可以有多久呢？
