Product Specifications:
Item#1091-G:rgp41 HIV-1 MN Conjugated to Colloidal Gold
Concentration: 25ug protein
Volume/vial: 1ml Gold Conjugate
Diluent: 10mM Tris, pH 8; 5% BSA; 0.05 PEG; 0.02% NaN3
Storage: 4°C
Stability: At least 3 months at 4°C Minimum
Dilution: 1 : 50
Application: HIV-1 Diagnostics, Antigen Capture, Dot Blot Assay-Anti-gp41 Antibody Binding, Immunohistochemistry.
Description: rgp41 HIV-1 MN conjugated to Colloidal Gold (45nm mean particle size).
Coupling Ratio: OD 520nm = 10; protein content approximately 25µg.
Specificity: Gold conjugated rgp41 binds to murine monoclonal antibodies of defined epitope specificity and human polyclonal antibodies as determined by dot blot assays.
Biological Activity: Not determined.
Application and Instructions for use:
Recommended dilutions for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. 1. Dot blot assays may be performed in 1 : 50 dilution range depending on the concentration of solid phase antibody in the test system. Antigen capture assays are performed with solid phase antigen in concentrations of 1ug or more using HIV-1 converted serum for antigen cross linking. 2. Immunohistochemical studies may be performed in 1 : 50 dilution range.
Related Products:
Item#: 1091-P Peroxidase-Conjugated rgp41 HIV-1 MN
Application: Human diagnostics.
Item#: 1091-F Flourescene-Conjugated rgp41 HIV-1 MN
Application: FACS analysis, Histocytology.
Item#: 1091-M Magnetically-Coupled rgp41 HIV-1 MN
Application: Human diagnostics, Human B and T-cell panning
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目前选择这个都是北京博凌科为生物科技有限公司的
在官方网上查到的可用产品信息如下。Leupeptin用水溶解，工作液有效浓度是10-100uM.我认为这个数值太大,我怎么算也不知道该加多少量..
PMSF用5%乙醇溶解，有效浓度没有说。怎样配,有效浓度应该是多少?
使用过的同学,帮忙讲几句.
