One-Step RT-PCR Kit
- Single-tube reaction
- Convenient
- Extremely sensitive to low-copy target RNA
- Successful amplification up to 12 kb or more
- Reproducible RNA amplification
Products
| Cat.No. | Product | Size |
|---|---|---|
| ORTHT100 | HelixCript™ One-Step RT-PCR Kit [Hot-Taq] | 100 rxns |
| ORTHT500 | HelixCript™ One-Step RT-PCR Kit [Hot-Taq] | 500 rxns |
| ORTA100 | HelixCript™ One-Step RT-PCR Kit [Ab+Taq] | 100 rxns |
| ORTA500 | HelixCript™ One-Step RT-PCR Kit [Ab+Taq] | 500 rxns |
| ORTH100 | HelixCript™ One-Step RT-PCR Kit [HyperSense-Taq] | 100 rxns |
| ORTH500 | HelixCript™ One-Step RT-PCR Kit [HyperSense-Taq] | 500 rxns |
| ORTS100 | HelixCript™ One-Step RT-PCR Kit [Speed-Pfu] | 100 rxns |
| ORTS500 | HelixCript™ One-Step RT-PCR Kit [Speed-Pfu] | 500 rxns |
- Description
- Data
- Details
- Documents
HelixCript™ One-Step RT-PCR Kit is designed for sensitive amplification of the target gene in one-tube reaction from total transcripts. A reverse transcriptase, HelixCript™ Thermo Reverse Transcriptase, and a thermostable DNA polymerase,
HelixAmp™ HyperSense-Taq polymerase, HelixAmp™ Speed-Pfu polymerase, or HelixAmp™ Hot-Taq polymeraseare supplied as an enzyme mixture.
You can choose on of the enzymes according to your purpose.
One-Step RT-PCR Kit [Hot-Taq] : High-specific RT-PCR
One-Step RT-PCR Kit [Ab+Taq]
One-Step RT-PCR Kit [HyperSense-Taq] : Long-range and high yield RT-PCR with low copy of target
One-Step RT-PCR Kit [Speed-Pfu] : Fast and high fidelity RT-PCR
One-step RT-PCR system provide the several advantages.
- Synthesis of cDNA and PCR amplification corresponding to target gene in one-tube reaction.
- Obtain the reproductive data in the repetitive experiment(s)
- Can save the time and cost for preparation of RT-PCR
- Amplification of low-copy transcripts by RT-PCR
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为了使供输血用的血液能保存一定时间,加入适当的抗凝剂,并尽量防止在保存期发生变质而用的液体。为了防止因糖的消失引起活力降低,尚加入葡萄糖。ACD液(含柠檬酸三钠、柠檬酸、葡萄糖)一般用于血库。添加ACD的血液放在4—6℃条件下保存,可保存三周。另外为防止柠檬酸盐的过量可用加磷酸钠的CPD液。此外还设计了加乳酸钠的ACDL液。如果加入甘油等防冻剂则可长时间冷冻保存红细胞。
目前选择这个都是北京博凌科为生物科技有限公司的
目前正在从新鲜病人肿瘤组织标本中提取细胞悬液进行阳性细胞的筛选,但由于实验室距离医院有3个小时的车程,请问什么储存方法或者储存液能短期保证肿瘤组织中细胞完整性?谢谢



