| Product Name | Calcein, AM *UltraPure Grade*, 5 mM solution in anhydrous DMSO |
| CAS | 148504-34-1 |
| Size | 200 µL |
| Catalog # | AS-89203 |
| US$ | $186 |
| Purity | ≥95% by HPLC |
Calcein, AM is a cell-permeant and non-fluorescent compound that is widely used for determining cell viability.In live cells the non-fluorescent calcein, AM is hydrolyzed by intracellular esterases into the strongly green fluorescent anion calcein. The fluorescent calcein is well-retained in the cytoplasm in live cells.Hela cells stained with AnaSpecs Calcein AM fluorescent dye (Cat# AS-89201, AS-89202, AS-89203 and AS-89204). MCF-7 cells grown in 8-well slide chamber for 2 days. Cells were washed twice before adding 1 uM Calcein AM (AnaSpec cat# AS-89202). Images acquired every 3 seconds and total elapsed time on video is 12 min.
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| Images | ![]() |
| Detailed Information | |
| Storage | -20°C desiccated and protected from light |
| Molecular Weight | 994.857 |
| Molecular Formula | C46H46N2O23 |
| Spectral Properties | Abs/Em = 494/517 nm |
| Solvent System | DMSO |
| Product Citations | Robinson, M. et al. (2011). Screening of hepatitis C virus inhibitors using genotype 1a HCV replicon cell lines. Curr Prot Microbiol doi: 10.1002/9780471729259.mc1707s22.Delaunay, JL. et al. (2009). A missense mutation in ABCB4 gene involved in progressive familial intrahepatic cholestasis type 3 leads to a folding defect that can be rescued by low temperature. Hepatology 49, 1218.Zhang, K-H. et al. (2009). Ferritin heavy chainmediated iron homeostasis and subsequent increased reactive oxygen species production are essential for epithelial-mesenchymal transition. Cancer Res. 10.1158/0008-5472.CAN-09-0112. |
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为了使供输血用的血液能保存一定时间,加入适当的抗凝剂,并尽量防止在保存期发生变质而用的液体。为了防止因糖的消失引起活力降低,尚加入葡萄糖。ACD液(含柠檬酸三钠、柠檬酸、葡萄糖)一般用于血库。添加ACD的血液放在4—6℃条件下保存,可保存三周。另外为防止柠檬酸盐的过量可用加磷酸钠的CPD液。此外还设计了加乳酸钠的ACDL液。如果加入甘油等防冻剂则可长时间冷冻保存红细胞。
目前选择这个都是北京博凌科为生物科技有限公司的
目前正在从新鲜病人肿瘤组织标本中提取细胞悬液进行阳性细胞的筛选,但由于实验室距离医院有3个小时的车程,请问什么储存方法或者储存液能短期保证肿瘤组织中细胞完整性?谢谢



