| Source | Human 293 Cells | M.W. | 37-40 kDa | CAS No. | |
|---|---|---|---|---|---|
| Structural Info | |||||
| Formulation | Lyophilized in sterile filtered solution of PBS. | ||||
| Reconstitution | Before reconstitution, a brief spin is recommend todrive down any material dislodged from the bottom of the tube. The lyophilized protein should bereconstituted in sterile H2O to a desired concentration. | ||||
| Stability | The lyophilized protein is stable for at least oneyear if stored at -80 °C. Reconstituted protein is stable for at least four weeks at 4 °C,but should be stored in aliquots at -80 °C for longer term. Avoidrepeated freeze and thaw. | ||||
| Purity | Greater than 90% as determined by SDS-PAGE analysis | ||||
| Biological Activity | The activity was determined by using a TCF reporter gene assay in cultured human cells.The IC50 ranges from 100 - 500 ng/ml in the inhibition of 100 ng/mL WNT-3a activity.Activity in other assays | ||||
| Country of Origin | USA | ||||
DKK-1, Dickkopf-related protein-1, is a member of the DKKprotein family which includes DKK-1 through DDK-4. DKK was originally identified as ahead-forming molecule in Xenopus. Mechanisticstudies showed that DKK-1 inhibits the Wnt /β-catenin signaling pathway byforming inhibitory complexes with co-receptor LRP5/6. Inhibition of Wnt/β-catenin signaling isessential for posterior patterning and anterior development in vertebrates, supportedby the discovery that DKK-1 knock-out mice lack head formation. StemRDexpressed recombinant human DKK-1 in human 293 cells as a 37-40 kDaglycoprotein containing 235 amino-acid residues. It is purified by a series of chromatography including HPLC.
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反应底物——葡萄糖
反应产物——ATP、乙醇、CO2或ATP、乳酸
有氧呼吸:
反应底物——葡萄糖
反应产物——ATP、CO2、H2O
ELISA实验,操作的时候,加入底物,溶液变成蓝色,孵育一段时间后,加入终止液,变成黄色,测吸光值。然后,当天忘记扔板子了,第二天想到去扔的时候,所有孔都变成无色透明的了!!!为什么会褪色???是试剂有问题?还是因为室温太高环境影响?如果是试剂问题那我之前做的结果还能信么?
调节至最适合温度(一般是37度),合适的酸碱度时,酶的活性最高
向左转|向右转向左转|向右转向左转|向右转向左转|向右转

