
Source | Human 293 Cells | M.W. | 37-40 kDa | CAS No. | |
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Structural Info | |||||
Formulation | Lyophilized in sterile filtered solution of PBS. | ||||
Reconstitution | Before reconstitution, a brief spin is recommend todrive down any material dislodged from the bottom of the tube. The lyophilized protein should bereconstituted in sterile H2O to a desired concentration. | ||||
Stability | The lyophilized protein is stable for at least oneyear if stored at -80 °C. Reconstituted protein is stable for at least four weeks at 4 °C,but should be stored in aliquots at -80 °C for longer term. Avoidrepeated freeze and thaw. | ||||
Purity | Greater than 90% as determined by SDS-PAGE analysis | ||||
Biological Activity | The activity was determined by using a TCF reporter gene assay in cultured human cells.The IC50 ranges from 100 - 500 ng/ml in the inhibition of 100 ng/mL WNT-3a activity.Activity in other assays | ||||
Country of Origin | USA |
DKK-1, Dickkopf-related protein-1, is a member of the DKKprotein family which includes DKK-1 through DDK-4. DKK was originally identified as ahead-forming molecule in Xenopus. Mechanisticstudies showed that DKK-1 inhibits the Wnt /β-catenin signaling pathway byforming inhibitory complexes with co-receptor LRP5/6. Inhibition of Wnt/β-catenin signaling isessential for posterior patterning and anterior development in vertebrates, supportedby the discovery that DKK-1 knock-out mice lack head formation. StemRDexpressed recombinant human DKK-1 in human 293 cells as a 37-40 kDaglycoprotein containing 235 amino-acid residues. It is purified by a series of chromatography including HPLC.
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酶促反应速率跟底物浓度,pH,温度,酶的 浓度等因素有关,当底物浓度足够高时,酶的浓度相对较低,酶只能与一部分底物结合,导致速率有一个最大值.
不同的酶Km值不同,同一种酶与不同底物反应Km值也不同,Km值可近似的反应酶与底物的亲和力大小:Km值大,表明亲和力小;Km值小,表明亲合力大。
Km最小的那个底物,就是酶的最适底物。
决定酶促反应最大速度Vm的因素是什么?是同一底物,尽管加不同种酶,其Vmax都相同吗?可以画曲线图解释一下吗?可是根据米曼氏方程来看又有点矛盾,Km变大,Vmax也需变大,才可使v变大。所以题目所述到底应该怎么理解呢

