
Source | Human 293 Cells | M.W. | 37-40 kDa | CAS No. | |
---|---|---|---|---|---|
Structural Info | |||||
Formulation | Lyophilized in sterile filtered solution of PBS. | ||||
Reconstitution | Before reconstitution, a brief spin is recommend todrive down any material dislodged from the bottom of the tube. The lyophilized protein should bereconstituted in sterile H2O to a desired concentration. | ||||
Stability | The lyophilized protein is stable for at least oneyear if stored at -80 °C. Reconstituted protein is stable for at least four weeks at 4 °C,but should be stored in aliquots at -80 °C for longer term. Avoidrepeated freeze and thaw. | ||||
Purity | Greater than 90% as determined by SDS-PAGE analysis | ||||
Biological Activity | The activity was determined by using a TCF reporter gene assay in cultured human cells.The IC50 ranges from 100 - 500 ng/ml in the inhibition of 100 ng/mL WNT-3a activity.Activity in other assays | ||||
Country of Origin | USA |
DKK-1, Dickkopf-related protein-1, is a member of the DKKprotein family which includes DKK-1 through DDK-4. DKK was originally identified as ahead-forming molecule in Xenopus. Mechanisticstudies showed that DKK-1 inhibits the Wnt /β-catenin signaling pathway byforming inhibitory complexes with co-receptor LRP5/6. Inhibition of Wnt/β-catenin signaling isessential for posterior patterning and anterior development in vertebrates, supportedby the discovery that DKK-1 knock-out mice lack head formation. StemRDexpressed recombinant human DKK-1 in human 293 cells as a 37-40 kDaglycoprotein containing 235 amino-acid residues. It is purified by a series of chromatography including HPLC.
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决定酶促反应最大速度Vm的因素是什么?是同一底物,尽管加不同种酶,其Vmax都相同吗?可以画曲线图解释一下吗?可是根据米曼氏方程来看又有点矛盾,Km变大,Vmax也需变大,才可使v变大。所以题目所述到底应该怎么理解呢
调节至最适合温度(一般是37度),合适的酸碱度时,酶的活性最高
我是新手,来到丁香园看到有这么多的热心人,感到很高兴!!我也有很多问题需要大家的帮助!!实验已经作了一年了可是毫无进展,心里很是着急!!
我的课题是以外消旋的苯基乙二醇为底物,用假丝酵母催化生成手性纯的S-型苯基乙二醇,由于是老课题,所以目标是提高转化反应的底物浓度和菌体的使用批次(目前菌体使用一批后便不能再使用)。
我曾试过很多种方法,但均效果不大!我试着在转化过程中添加醛类,酮类,醇类作为辅助底物,增加菌体的使用批次。可效果不好,特别是添加了醇类后还有的起了反作用,因为我的这个转化过程中涉及到NAD和NADPH的再生。(其转化过程是酵母先催化将外消旋的苯基乙二醇变为酮,再将酮还原为醇,经过这一过程就将外消旋的苯基乙二醇变为手性纯的S-型了)
我还试过用固定化的方法,海藻酸钙包埋法,可是这样底物浓度就更低了!!
我现在不知道下一步该如何做了,很着急,请大家帮帮忙。谢谢了!!谢谢
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