DESCRIPTION:In reactions dependent on its co-substrate NAD, SIRT5 catalyzes the desuccinylation/demalonylation of protein acylated lysine residues and, far less efficiently, lysine deacetylation. SIRT5, like SIRTs 3 and 4, is located in the mitochondria (residues 1-36 encode a presumptive transit sequence), although some reports indicate additional localization to the cytoplasm, possibly of isoform 1 specifically. Recent proteomic studies reveal relatively widespread lysine succinylation, which, at least in the mitochondrial matrix, may result from non-enzymatic reaction of lysyl amines with succinyl-CoA. SIRT5 expression levels can be elevated by calorie restriction or fasting and metabolic regulatory functions for both SIRT5 desuccinylation and deacetylation activities have been proposed. Activation of the urea cycle enzyme carbamoyl phosphate sythetase 1 (CPS1) by SIRT5-catalyzed deacetylation and of SOD1 (superoxide dismutase 1) by SIRT5-catalyzed desuccinylation have been demonstrated. As in the case of SIRT1, SIRT5 deacetylation activity (but not desuccinylation) can be activated by certain polyphenols such as resveratrol or isoliquiritigenin, although at ~10x higher concentrations. A crystal structure of SIRT5 in complex with resveratrol and a fluorigenic acetylated peptide substrate has been obtained and is the basis of a proposed activation mechanism in which resveratrol binds to form a “top cover” over the peptide binding cleft and interacts with the peptide substrate C-terminal to the acetyllysine. However, given the absence in SIRT5 of structures homologous to the N-terminal domain that enhances SIRT1 catalytic efficiency and to the conserved, structured region and residue (E230) important for full SIRT1 activation by resveratrol and other compounds, it is still unclear to what extent this model will be applicable to SIRT1.
ACCESSION #: NM_012241
Uniprot Link
INCLUDES AMINO ACIDS: 37-310 (C-term.)
TAG(S): N-terminal His-tag
MW: 32.3 kDa
EXPRESSION SYSTEM: E. coli
SUPPLIED AS: Solution of purified recombinant protein in 50 mM Tris/HCl pH 7.5, 500 mM NaCl, 1 mM TCEP, 10% glycerol (v/v).
STORAGE: -80°C, aliquot and snap-freeze after first use.
SIZE:100ug
View Product Data Sheet
Substantial Discounts Available on Bulk Quantities
We also offer assay services for SIRT5.
eactionBIOLOGy公司成立于2001年，是专门从事早期药物发现化验服务的公司，提供广泛的服务，包括用于激酶和表观遗传生物化学以及基于细胞的高通量筛选（HTS）和选择性化合物分析的稳定测定技术。我们还提供相关的HTS检测试剂，包括高度纯化和特征化的表观遗传蛋白。平均每年进行数百万次HTS和性能分析。在全球有超过750家公司（包括制药，生物技术，学术和非营利实验室）购买我们的产品。
MedicalDeviceDepot，Inc.（MDD）的成立基于一个简单的想法：改变全球医疗保健专业人员购买其医疗设备的方式。许多医疗设备供应商的员工常常缺乏充分满足客户需求并回答所有问题所需的培训和知识。对于繁忙的医师和其他寻求购买优质医疗设备的医疗保健专业人员而言，结果通常是一个缓慢，麻烦且令人沮丧的过程。医疗器械仓库有所不同。我们经验丰富的销售和服务代表经过全面培训，可以回答您有关我们广泛目录中产品的所有问题。我们的首要任务是确保您对每次购买都充满信心。我们致力于为您提供无忧的愉快体验。使用医疗设备仓库时，您可以期望得到以下结果：来自礼貌，知识渊博的代表的个性化服务-您将永远不会使用机器。如果您的代表在呼叫时与另一个客户一起工作，则在一个小时内返回的呼叫。每次互动中的诚实和透明。我们珍惜您的时间，并了解购买医疗设备时您有很多选择。医疗器械仓库保证了响应，高效，周到的服务，这是我们对您的承诺。我们期待着帮助您满足所有医疗设备需求。
ReactionBiology详细产品列表：货号产品价格（美元）HMT-11-376ASH1L(His)Methyltransferase325DMT-21-124DNMT1450DMT-21-125DNMT3aMethyltransferase450DMT-21-126 DNMT3b450HMT-11-101DOT1LMethyltransferase395HMT-25-115 EZH1Complex450HMT-25-114EZH2Complex799HMT-25-173EZH2-Y641FComplex799HMT-11-102G9aMethyltransferase(GST)(aa786-1210)299HMT-11-245G9aMethyltransferase(His)(aa913-1193)299HMT-12-104G9a/GLPComplexMethyltransferase325HMT-11-103GLPMethyltransferaseProtein299HMT-15-105MLL1Complex499HMT-15-106 MLL2Complex499HMT-15-107MLL3Complex499HMT-15-108 MLL4Complex499MT-11-359NNMT(GST)(NicotinamideN-Methyltransferase)299MT-11-358NNMT(His)(NicotinamideN-Methyltransferase)299HMT-11-319NRMT1(GST)325HMT-21-139NSD1Methyltransferase450HMT-21-1380NSD2(His)Methyltransferase450HMT-21-122 NSD2Methyltransferase550HMT-11-377NSD2SET(His)325HMT-21-159NSD2-E1099KMethyltransferase599HMT-11-378NSD2-E1099K-SET(His)325HMT-21-181NSD2-T1150AMethyltransferase450HMT-11-379NSD2-T1150A-SET(His)325HMT-11-132 NSD3Methyltransferase325HMT-21-348PRDM2Methyltransferase325HMT-21-152PRDM9Methyltransferase450HMT-11-119PRMT1Methyltransferase325HMT-11-113PRMT3Methyltransferase325HMT-11-120PRMT4Methyltransferase399HMT-21-172PRMT5Methyltransferase450HMT-22-434PRMT5(C449S)/MEP50799HMT-22-148PRMT5/MEP50Complex799HMT-11-121PRMT6Methyltransferase(GST)325HMT-21-380PRMT6Methyltransferase(His)325HMT-21-382PRMT7(His)450HMT-11-135PRMT8Methyltransferase325MT-11-356s-COMT(His)(SolubleCatechol-O-Methyltransferase)299MT-11-357s-COMT(V108M)(His)(SolubleCatechol-O-Methyltransferase）299HMT-15-116 SET1AComplex(5-subunits)499HMT-15-117SET1BComplex(5-subunits)499HMT-11-133SET7/9Methyltransferase299HMT-11-476SET8(GST)299HMT-11-118 SET8Methyltransferase299HMT-11-128 SETD2(GST)Methyltransferase299HMT-11-129SETD2(His)Methyltransferase299HMT-11-110SMYD2Methyltransferase325HMT-11-111SUV39H1Methyltransferase299HMT-11-418SUV39H2(GST)Methyltransferase299HMT-11-112SUV39H2(His)Methyltransferase299HMT-21-149SUV420H1-tv1Methyltransferase450HMT-21-150SUV420H1-tv2Methyltransferase450HMT-21-349SUV420H2Methyltransferase325HMT-14-109WRAD2375HMT-14-438(H3.3-H4)2Tetramer325HMT-35-435CoreHistones(Chicken)250HMT-11-184Fibrillarin(GST)325HMT-11-183Fibrillarin(His)325HMT-11-137GST-GAR299HMT-12-316H2A/H2Bdimers325HMT-11-180HistoneH1.050HMT-11-146HistoneH2A50HMT-11-147HistoneH2B50HMT-11-134 HistoneH3.350HMT-35-179Nucleosomes(ChickenMono/Di)280HMT-35-182Nucleosomes(ChickenOligo/PolyH5Enriched)280
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ELISA实验，操作的时候，加入底物，溶液变成蓝色，孵育一段时间后，加入终止液，变成黄色，测吸光值。然后，当天忘记扔板子了，第二天想到去扔的时候，所有孔都变成无色透明的了！！！为什么会褪色？？？是试剂有问题？还是因为室温太高环境影响？如果是试剂问题那我之前做的结果还能信么？
不同的酶Km值不同，同一种酶与不同底物反应Km值也不同，Km值可近似的反应酶与底物的亲和力大小：Km值大，表明亲和力小；Km值小，表明亲合力大。
Km最小的那个底物，就是酶的最适底物。
我是新手，来到丁香园看到有这么多的热心人，感到很高兴！！我也有很多问题需要大家的帮助！！实验已经作了一年了可是毫无进展，心里很是着急！！
我的课题是以外消旋的苯基乙二醇为底物，用假丝酵母催化生成手性纯的S-型苯基乙二醇，由于是老课题，所以目标是提高转化反应的底物浓度和菌体的使用批次（目前菌体使用一批后便不能再使用）。
我曾试过很多种方法，但均效果不大！我试着在转化过程中添加醛类，酮类，醇类作为辅助底物，增加菌体的使用批次。可效果不好，特别是添加了醇类后还有的起了反作用，因为我的这个转化过程中涉及到NAD和NADPH的再生。（其转化过程是酵母先催化将外消旋的苯基乙二醇变为酮，再将酮还原为醇，经过这一过程就将外消旋的苯基乙二醇变为手性纯的S-型了）
我还试过用固定化的方法，海藻酸钙包埋法，可是这样底物浓度就更低了！！
我现在不知道下一步该如何做了，很着急，请大家帮帮忙。谢谢了！！谢谢
