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GenWay/Clostridium tetani toxin 5S IgG quantitative/GWB-1858E0/1x96 Assays
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4000-520-616
Description:
PleaseclickhereforMSDSPDFDatasheetProductName:Clostridiumtetanitoxin5SIgG-ELISA
#ofSamples:1x96Assays
IntendedUse:TheClostridiumtetanitoxin5SIgG-ELISAisintendedforthequantitativedeterminationofIgGclassantibodiesagainstClostridiumtetanitoxininhumanserumorplasma(citrate).Thisallowsforthedeterminationoftheimmunestatusofthepatientsfacilitatingindividualrecommendationsaboutthenecessityofabasicimmunizationorboosterinjection.
Introduction:Clostridiaarespore-forminggram-positivebacteria.Theroundsporesarebuildattheterminalendwhichresultsinthemicroscopeinatennisracketlikeshape.
TetanusdevelopsonlywhensporesofClostridiumtetanigerminateunderstrictanaerobicconditionsaftergainingaccesstowoundsandsmalllacerations.Theclinicalmanifestationofthediseaseisprimarynotcausedbytheinvasionoftheexciterbutbythesecretionofapowerfulneurotoxin(tetanospasmin).Thistoxinblockstheinhibitionofthesignaltransductionandhasahighaffinitytothecentralnervoussystem.TheconsequenceishyperexcitABIlityofthemusclestoexternalstimuliincombinationwithaprincipalincreaseofthemuscletonuswithoutinfluenceofconsciousness.Itstartswithtonicspasmofmuscles(trimus),mimicmusclesandgalletmuscles.Neck,backandaBDominalmusculaturefollow.Atthesametimetheappearanceofrefectoryspasmofwholemusclegroupscanhamperbreathing.Hypersalivationandswallowingproblemscauseaspirationandpneumoniawiththenextbreath.
Clostridiumtetaniisubiquitouspresentinsoilandintestineofhumansandanimals.Ingestionofbacteriaorgrowthintheintestineofmanoranimaliswithoutharm.Thesporesareextremelyresistanttowardsheatandcanstayinfectiousforalongperiod.Thebacteriacangetundertheskinbyevensmallestwounds.InEuropetetanusmainlyoccursafterinjuriesandsometimespostoperativewhereasindevelopingcountriesTetanusiswidelydisseminated.TheWHOassumesthatonemillionpeoplediebecauseoftetanusworldwideperyear.
Tetanustoxinisanexcellentimmunogeninman-onlyoneantigenictypeoftoxin.Theonlyeffectivewaytocontroltetanusisbyprophylacticactiveimmunization.
Infectionmaybeidentifiedby
Microscopy:Gramstain
SEROlogy:DetectionoftoxinproductionbyELISA
Principlesoftheassay:ThequantitativeimmunoenzymaticdeterminationofIgG-classantibodiesagainstTetanustoxoidisbasedontheELISA(Enzyme-linkedImmunosorbentAssay)technique.
MicrotiterstripwellsareprecoatedwithinactivatedTetanustoxoidantigenstobindcorespondingantibodiesofthespecimen.AfterwashingthewellstoremoveallunboundsamplematerialhorserADIshperoxidase(HRP)labelledanti-humanIgGconjugateisadded.ThisconjugatebindstothecapturedTetanustoxoidspecificantibodies.TheimmunecomplexformedbytheboundconjugateisvisualizedbyaddingTetramethylbenzidine(TMB)substratewhichgivesabluereactionproduct.
TheintensityofthisproductisproportionaltotheamountoftoxoidspecificIgGantibodiesinthespecimen.Sulphuricacidisaddedtostopthereaction.Thisproducesayellowendpointcolour.Absorbanceat450nmisreadusinganELISAmicrowellplatereader.
StorageandStability:Thereagentsarestableuptotheexpirydatestatedonthelabelwhenstoredat2...8°C.
LimitationsoftheTest:Bacterialcontaminationorrepeatedfreeze-thawcyclesofthespecimenmayaffecttheabsorbancevalues.Diagnosisofaninfectiousdiseaseshouldnotbeestablishedonthebasisofasingletestresult.Aprecisediagnosisshouldtakeintoconsiderationclinicalhistory,symptomatologyaswellasserologicaldata.
Inimmunocompromizedpatientsandnewbornsserologicaldataonlyhaverestrictedvalue.
ReferencesAmbrosch,F.,Wiedermann,G.,Müller,H.:EineneueMikro-ELISA-MethodezurBestimmungderTetanus-Antikörper.Zbl.Bakt.Hyg.A258,173-182(1984)Mai,K.,Bartelheimer,H.K.,Rosin,H.:ÜberdenStandunddieDauerdesImpfschutzesgegenTetanusbeiKindern.Dtsch.med.Wschr.95,1044(1970)Müller,H.E.,Müller,M.,Schiek,W.:Tetanus-Schutzimpfung-IndikationundKontraindikation.Dtsch.med.Wschr.113,1326(1988)Pietsch,M.:ImpfserologiezurErgänzungvonImpfungen.Allgemeinarzt15,1155-1156(1993)Schröder,J.P.,Kuhlmann,W.D.:TetanusimmunitätbeiMännernundFraueninderBRD.Immun.Infekt.19,14(1991)
AdditionalInformation:
| Name | Clostridiumtetanitoxin5SIgGquantitative |
|---|---|
| RelatedProductNames | Clostridiumtetanitoxin5SIgG-ELISA |
| MolecularWeight | 0.5 |
| Storage | Thereagentsarestableuptotheexpirydatestatedonthelabelwhenstoredat2...8°C. |
| IntendedUse | ResearchUseOnly |
