
Description:
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ProductName:TBE/FSMEIgGplus-ELISA
#ofSamples:1x96Assays
IntendedUse:TheTBE/FSMEIgGplus-ELISAisintendedforthequantitativedeterminationofIgGclassantibodiesagainstTBEvirusinhumanserumorplasma(citrate).
Introduction:Tick-borneencephalitis(TBE)virusisaflavivirusofthefamilyTogaviridae.Itisanenvelopedsingle-strandedRNAviruswithcubicicosahedralsymmetryandrangesinsizefrom20-80nmindiameter.
OntheEuropeancontinentonlytwoantigenicsubtypesexistwhichshowonlylittledifferencesintheirstructuralproteins.
TBEvirusismainlytransmittedbyticks.Thedegreeofcontaminationofticks(andthushumans)incentralEuropeincreasesfromwesttoeast,andanybodymaybeaffected.Specificantibodydevelopmentyieldsalife-longimmunity.
TBEisthemostimportanttick-transmitteddiseaseofman-besideLymedisease,whichiscausedbythespiRocheteBorreliaburgdorferi.Theclinicalcourseofthediseasedependsontheimmunestatusoftheinfectedpersons.Ahighvirusproductionintheprimaryinfectedtissuesisrequiredforthepassageoftheblood-brainbarrierandtheresultingseveremanifestationsinthecentralnervoussystem.
Ofthetwosubtypes,RSSEcausesthemoresevereinfection,havinganincidenceofmortalityofupto25%insomeoutbreaks,whereasmortalityinCEEseldomexceeds5%.AninactivatedTBEvirusvaccineisavailableinEuropeandRussia.Thepresenceofvirusresp.infectionmaybeidentifiedby:
Haemagglutination-Inhibition,ComplementFixation
DetectionofspecificantibodiesbyCIE,ELISA
Principlesoftheassay:ThequantitativeimmunoenzymaticdeterminationofIgG-classantibodiesagainstTBEVirusisbasedontheELISA(Enzyme-linkedImmunosorbentAssay)technique.
MicrotiterstripwellsareprecoatedwithTBEVirusantigenstobindcorrespondingantibodiesofthespecimen.AfterwashingthewellstoremoveallunboundsamplematerialhorserADIshperoxidase(HRP)labelledanti-humanIgGconjugateisadded.ThisconjugatebindstothecapturedTBEVirusspecificantibodies.TheimmunecomplexformedbytheboundconjugateisvisualizedbyaddingTetramethylbenzidine(TMB)substratewhichgivesabluereactionproduct.
TheintensityofthisproductisproportionaltotheamountofTBEVirusspecificIgGantibodiesinthespecimen.Sulphuricacidisaddedtostopthereaction.Thisproducesayellowendpointcolour.Absorbanceat450nmisreadusinganELISAmicrowellplatereader.
StorageandStABIlity:Thereagentsarestableuptotheexpirydatestatedonthelabelwhenstoredat2...8°C.
LimitationsoftheTest:Crossreactivitywithantibodiesofotherflaviviruses,forexampleDengueVirus,Yellowfever,Japaneseencephalitisviruses,cannotbeexcluded.
Bacterialcontaminationorrepeatedfreeze-thawcyclesofthespecimenmayaffecttheabsorbancevalues.Diagnosisofaninfectiousdiseaseshouldnotbeestablishedonthebasisofasingletestresult.Aprecisediagnosisshouldtakeintoconsiderationclinicalhistory,symptomatologyaswellasSEROlogicaldata.Inimmunocompromizedpatientsandnewbornsserologicaldataonlyhaverestrictedvalue.
ReferencesRoggendorfM.Frühsommer-MeningoenzephalitisWersollgeimpftwerden?Therapiewoche,40,1173(1990)RoggendorfM.etal.SerologicalDiagnosisofAcuteTick-BorneEncephalitisbyDemonstrationofAntibodiesoftheIgMclass.J.Med.Virol.,7,41(1981)HofmannH.etal.RapiddiagnosisofTick-BorneEncephalitisbyMeansofEnzymeLinkedImmunosorbentAssay.J.Gen.Virol.,42,505(1979)HofmannH.etal.ELISAforIgMAntibodiesagainstTick-BorneEncephalitisVirus:QuantificationandStandardizationofResults.Zbl.Bakt.I.Orig.,255,448(1983)Tick-BorneEncephalitis(TBE)anditsImmunoprophylaxis,IMMUNOAG,Wien(1997)
AdditionalInformation:
Name | TBE/FSMEIgGplusquantitative |
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RelatedProductNames | TBE/FSMEIgGplus–ELISA |
MolecularWeight | 0.5 |
Storage | Thereagentsarestableuptotheexpirydatestatedonthelabelwhenstoredat2...8°C. |
IntendedUse | ResearchUseOnly |