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GenWay/C-Peptide/GWB-AE53DE/1x96 Assays
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4000-520-616
Description:
ProductName:C-Peptide#ofSamples:1x96Assays
IntendedUse:ImmunoenzymaticcolorimetricmethodforquantitativedeterminationofC-Peptideinhumanserum.
Introduction:C-peptideistheabbreviationforconnectingpeptide;itisa31-amminoacidpeptide.C-peptideofinsulinistheC-terminalcleavageproductproducedduringprocessingoftheinsulinprohormonetothematureinsulinmolecule.Proinsuliniscleavedwhenitisreleasedfromthepancreasintotheblood-oneC-peptideforeachinsulinmolecule.C-PeptideisdevoidofanyBIOLOGicalactivitybutappearstobenecessarytomaintainthestructuralintegrityofInsulin.
In-vitrodeterminationofInsulinandC-Peptidelevelhelpindifferentialdiagnosisofliverdisease,acromegaly,Cusingsyndrome,familialglucoseintolerance,Insulinimia,renalfailure,ingestionofaccidentaloralhypoglycaemicdrugsorC-peptideinducedfactitioushypoglycaemia.
NewlydiagnoseddiabetespatientoftengettheirC-peptidelevelsmeasured,tofindiftheyhavetype1diabetesortype2diabetes.Thepancreasofpatientswithtype1diabetesisunabletoproduceinsulinandtheywillthereforeusuallyhaveadecreasedlevelofC-peptide,whileC-peptidelevelsintype2patientsisnormalorhigherthannormal.MeasuringC-peptideinpatientsinjectinginsulincanhelptodeterminehowmuchoftheirownnaturalinsulinthesepatientsarestillproducing.
C-peptideassaysmaybeanalyticallymoresensitivethaninsulinassays.MeasurementoftheC-peptidemaybeusefulinevaluatingendogenousinsulinsecretioninavarietyofclinicalconditions.InsulinandC-Peptidearesecretedintoportalcirculationinequimolarconcentrations;fastinglevelsofC-Peptideare510foldhigherthanthoseofInsulinowingtothelongerhalf-lifeofC-Peptide.TheliverdoesnotextractC-Peptidehowever;itisremovedfromthecirculationbydegradationinthekidneyswithafractionpassingoutunchangedinurine.HencetheurineC-PeptidelevelscorrelatewellwithfastingC-Peptidelevelsinserum
Principlesoftheassay:Inthismethod,C-Peptidecalibrators,patientspecimensand/orcontrolscontainingthenativeantigenarefirstaddedtostreptavidincoatedwells.BiotinylatedmonoclonalandhorserADIshperoxidase(HRP)labelledantibodiesareaddedandthereactantsaremixed.ThedifferenttypesofantibodiesusedhavehighaffinityandspecificityandaredirectedagainstdistinctanddifferentepitopesofC-Peptide.ReactionbetweenthevariousC-PeptideantibodiesandnativeC-Peptideoccursinthemicrowellswithoutcompetitionorsterichindranceformingasolublesandwichcomplex.
StorageandStABIlity:Theclosedreagentsarestableuptotheexpirydatestatedonthelabelwhenstoredat2...8°Cinthedark.
Openedreagentsarestablefor60dayswhenstoredat2?8°C.
LimitationsoftheTest:Bacterialcontaminationorrepeatedfreeze-thawcyclesofthespecimenmayaffecttheabsorbancevalues.
ReferencesEasthamR.D:BiochemicalValuesinClinicalMedicine,7thEd.Bristol.England.JonhWright&Sons,Ltd;.(1985).Gerbitz,V.K.D,J.Clin.Chem.Biochem.18,313-326(1980)BoehmTMandLebovitzHE.DiabetesCare479-490.(1979)NationalCommitteeforClinicalLaboratoryStandards.Procedureforthecollectionofdiagnosticbloodspecimensbyvenipuncture:approvedstandards.4thEd.NCCLSDocumentH3-A4,Wayne,PA(1988).TurkintonRW,etal.ArchiveofInternalMed.142,11021105(1982)SacksBD:CarbohydratesinBurtis,C.A.andAshwood,AR(Eds)TietzTextbookOFClinicalChemistry.2ndEd.Philadelphiaw.B.SaundersCo.1994KahnCR,eta.lDiabetesCare2,283295(1979)
AdditionalInformation:
Name | C-Peptide |
---|---|
RelatedProductNames | C-Peptide |
MolecularWeight | 0.5 |
Storage | Theclosedreagentsarestableuptotheexpirydatestatedonthelabelwhenstoredat2...8°Cinthedark. Openedreagentsarestablefor60dayswhenstoredat2?8°C. |
IntendedUse | ResearchUseOnly |