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GenWay/Anti Deamidated Gliadin Peptide (DGP) IgG/GWB-521207/1x96 Assays
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4000-520-616
Description:
| Introduction | Celiacdisease,alsoknownasglutensensitiveenteropathyisprimarilyadiseaseoftheinfantorganism.ItiscausedbyahypersensitivityreactioninresponsetogliADIn,aproteinbeingpresentinmanycereals.This,nonIgEmediatedfoodallergyleadstomassivemalabsorptiondisturbancesandischaracterizedbyacompleteatrophyofthevilliandahyperplasiaofthecryptsoftheupperintestine.Accordinglypatientssufferingfromceliacdiseasemustmaintainaglutenfreedietfortherestoftheirlife.Gliadinsareproteinscontaininghighamountsoftheaminoacidsprolinandglutamine.Theseproteinsbelongtothenutritivetissueofthegrainseedsofwheat,oat,barleyandryeandareresponsIBLeforthebakingpropertiesoftheflour.DuetothepossibilitiesofthehighlyspecificandsensitiveSEROlogicaldeterminationofIgGandIgGantibodiesagainstDGPtheinvasiveproceduresofbiopsiescanbegivenup.InthepastseveralbiopsieshavebeendonewithpatientswhenceliacdiseasewassUSPected,afteraperiodofagluten-freedietandalsoafteraspecificglutenchallenge.DGPantibodiestiterhasbeenprovedtocorrelateverywellwiththemorphologicalappearanceofthemucosaoftheupperintestine.IthasbeenwelldocumentedthatDGPantibodieslevelfallveryquicklyafteraglutenfreediethasbegunandriseimmediatelyafterrestoringglutentothediet.Thustheserologicaltestrepresentsareliablemethodtomonitorpatients,andinparticularchildrenandteenagers,fortheiradherencetothegluten-freediet. |
|---|---|
| IntendedUse | Anti-DeamidatedGliadinPeptide(DPG)IgGkitisasolidphaseenzymeimmunometricassay(ELISA)designedforthequantitativemeasurementofIgGclassantibodiesdirectedagainstdeamidatedGliadinpeptides(DGP)inhumanserumorplasma.Anti-DeamidatedGliadinPeptide(DPG)IgGisintendedforlaboratoryuseonly. |
| PrincipleoftheAssay | Anti-DeamidatedGliadinPeptide(DPG)IgGtestisbasedonthebindingofpresentantibodiesincalibrators,controlsorpredilutedpatientsamplesonthesynteticdeamidatedGliadinpeptides(DGP)coatedontheinnersurfaceofthewells.Aftera30minutesincubationthemicroplateiswashedwithwashbufferforremovingnon-reactiveserumcomponents.Ananti-human-IgGhorseradishperoxidaseconjugatesolutionrecognizesIgGclassantibodiesboundtotheimmobilizedantigens.Aftera30minutesincubationanyexcessenzymeconjugate,whichisnotspecificallyboundiswashedawaywithwashbuffer.AchromogenicsubstratesolutioncontainingTMBisdispensedintothewells.After15minutesofincubationthecolordevelopmentisstoppedbyaddingthestopsolution.Thesolutionscolorchangesintoyellow.TheamountofcolorisdirectlyproportionaltotheconcentrationofIgGantibodiespresentintheoriginalsample. |
AdditionalInformation:
| Name | AntiDeamidatedGliadinPeptide(DGP)IgG |
|---|---|
| RelatedProductNames | AntiDeamidatedGliadinPeptideImmunoglobulinGELISAKit,AntiDGPImmunoglobulinGELISA,AntiDGPIgGELISA,AntiDeamidatedGliadinPeptideIgGELISA,CeliacdiseaseELISA,GastroEnterologyAssay |
| MolecularWeight | 0.5 |
| Concentration | LotSpecific |
| Applications | Enzymeimmunoassayforthequantitativedeterminationofauto-antibodiesagainstdeamidatedGliadinpeptides(DGP)inhumanserumorplasma |
| Reactivity | Human |
| Storage | Storeallthekitreagentsat2-8°C.Donotfreeze.ReagentsarestableuntiltheexpirationdatewhenstoredandhandledasdirectedUnusedantigencoatedmicrowellstripsshouldberesealedsecurelyinthefoilpouchcontainingdesiccantsandstoredat |
| IntendedUse | ResearchUseOnly |

