![NewEast/Rab5B Activation Assay Kit [83401] [20 Assays]/83401](images/201910/1571694033440777269.jpg)
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ProductDescriptionSmallGTPasesareasuper-familyofcellularsignalingregulators.Rab5isamemberoftheRab-familyGTPases.Rab5isarate-limitingcomponentofthemachineryregulatingthekineticsofmembranetrafficintheearlyendocyticpathway. CurrentlythereisnodirectassaytomeasuretheactivationofRabGTPases. NewEastBiosciencesRab5BActivationAssayKitisbasedontheconfiguration-specificmonoclonalantibodythatspecificallyrecognizesRab5-GTP,butnotRab5-GDP,andaRab5Bspecificrabbitpolyclonalantibody.Giventhehighaffinityofmonoclonalantibodiestotheirantigens,theactivationassaycouldbeperformedinashorttime.Thisassayprovidesthereliableresultswithconsistentreproducibility. Thisanti-Rab5-GTPmonoclonalantibodycanalsobeusedtomonitortheactivationofRab5incellsandintissuesbyimmunohistochemistry. NewEastBiosciencesRab5BActivationAssayKitprovidesasimpleandfastmethodtomonitortheactivationofRab5B.Eachkitprovidessufficientquantitiestoperform20assays. AssayPrincipleNewEastBiosciencesRab5BActivationAssayKitbasesontheconfiguration-specificanti-Rab5-GTPmonoclonalantibodytomeasuretheactiveRab5B-GTPlevels,eitherfromcellextractsorfrominvitroGTPγSloADIngRab5Bactivationassays.Briefly,anti-activeRab5mousemonoclonalantibodywillbeincubatedwithcelllysatescontainingRab5-GTP.TheboundactiveRab5willthenbepulleddownbyproteinA/Gagarose.TheprecipitatedactiveRab5Bwillbedetectedbyimmunoblotanalysisusinganti-Rab5Bspecificrabbitpolyclonalantibody. ![]()
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