![NewEast/Rab5C Activation Assay Kit [83501] [20 Assays]/83501](images/201910/1571694009662861349.jpg)
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ProductDescriptionSmallGTPasesareasuper-familyofcellularsignalingregulators.Rab5isamemberoftheRab-familyGTPases.Rab5isarate-limitingcomponentofthemachineryregulatingthekineticsofmembranetrafficintheearlyendocyticpathway. CurrentlythereisnodirectassaytomeasuretheactivationofRabGTPases. NewEastBiosciencesRab5CActivationAssayKitisbasedontheconfiguration-specificmonoclonalantibodythatspecificallyrecognizesRab5-GTP,butnotRab5-GDP,andaRab5Cspecificrabbitpolyclonalantibody.Giventhehighaffinityofmonoclonalantibodiestotheirantigens,theactivationassaycouldbeperformedinashorttime.Thisassayprovidesthereliableresultswithconsistentreproducibility. Thisanti-Rab5-GTPmonoclonalantibodycanalsobeusedtomonitortheactivationofRab5incellsandintissuesbyimmunohistochemistry. NewEastBiosciencesRab5CActivationAssayKitprovidesasimpleandfastmethodtomonitortheactivationofRab5C.Eachkitprovidessufficientquantitiestoperform20assays. AssayPrincipleNewEastBiosciencesRab5CActivationAssayKitbasesontheconfiguration-specificanti-Rab5-GTPmonoclonalantibodytomeasuretheactiveRab5C-GTPlevels,eitherfromcellextractsorfrominvitroGTPγSloADIngRab5Cactivationassays.Briefly,anti-activeRab5mousemonoclonalantibodywillbeincubatedwithcelllysatescontainingRab5-GTP.TheboundactiveRab5willthenbepulleddownbyproteinA/Gagarose.TheprecipitatedactiveRab5Cwillbedetectedbyimmunoblotanalysisusinganti-Rab5Cspecificrabbitpolyclonalantibody. ![]()
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