![NewEast/Rab35 Activation Assay Kit [82801][20 Assays]/82801](images/201910/1571694009662861349.jpg)
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ProductDescriptionTheRabfamilyofGTPasesregulateseukaryoticvesicularmembranetraffic.Rab35iswithbothplasmamembraneandendosomallocalization,andhasbeenimplicatedindiverseprocessesthatincludeT-cellreceptorrecycling,oocyteyolkproteinrecyclingandcytokinesis.Rab35regulatesneuriteoutgrowthinneuronal-likecells,andcaninduceprotrusions. CurrentlythereisnodirectassaytomeasuretheactivationofRab35GTPases. NewEastBiosciencesRab35ActivationAssayKitisbasedontheconfiguration-specificmonoclonalantibodythatspecificallyrecognizesRab35-GTP,butnotRabRab35-GDP.Giventhehighaffinityofmonoclonalantibodiestotheirantigens,theactivationassaycouldbeperformedinashorttime.Thisassayprovidesthereliableresultswithconsistentreproducibility. Theseanti-Rab35-GTPmonoclonalantibodiescanalsobeusedtomonitortheactivationofRab35incellsandintissuesbyimmunohistochemistry. NewEastBiosciencesRab35ActivationAssayKitprovidesasimpleandfastmethodtomonitortheactivationofRab35.Eachkitprovidessufficientquantitiestoperform20assays. AssayPrincipleNewEastBiosciencesRab35ActivationAssayKitbasesontheconfiguration-specificanti-Rab35-GTPmonoclonalantibodytomeasuretheactiveRab35-GTPlevels,eitherfromcellextractsorfrominvitroGTPγSloADIngRab35activationassays.Briefly,anti-activeRab35mousemonoclonalantibodywillbeincubatedwithcelllysatescontainingRab35-GTP.TheboundactiveRab35willthenbepulleddownbyproteinA/Gagarose.TheprecipitatedactiveRab35willbedetectedbyimmunoblotanalysisusinganti-Rab35rabbitpolyclonalantibody. ![]()
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